Stimuli-responsive properties of peptide-based copolymers studied via directional growth of self-assembled patterns on solid substrate.
Stimuli-responsive properties of peptide-based copolymers studied via directional growth of self-assembled patterns on solid substrate.
复制标题
DOI：
10.1021/bm900368c
复制
发表时间：
2009-07-13
影响因子：
6.2
通讯作者：
Minko, Sergiy
中科院分区：
文献类型：
--
作者：
Sheparovych, Roman;Roiter, Yuri;Yang, Jiyuan;Kopecek, Jindrich;Minko, Sergiy
我们研究了基于肽的ABA和CBC三嵌段共聚物（通过细菌表达获得）的自组装，该共聚物含有随机卷曲的亲水性中心B嵌段，两侧是螺旋A或C嵌段。A和C嵌段相对于赖氨酸残基的部分具有不同的组成，这为共聚物溶液提供了更高的ph敏感性。研究了在外部刺激（pH和温度）的驱动下，共聚物溶液在固体基底上沉积的薄膜中进行大分子自组装过程中的链间相互作用。参与大分子结合的相互作用影响了发育模式的形态。B片段的多肽不参与二级结构的形成，而A和C片段表现出负责分子间结合的螺旋折叠。共聚物的响应机理是在pH值或温度变化时螺旋可逆组装成螺旋状结构。结果表明，在低pH条件下，当静电斥力较强，空调块展开时，组装产生分形枝晶。pH值的增加导致空调块体螺旋结构的恢复，并导致分形结构向致密结构的转变。温度的升高导致树枝状结构的破坏。本文报道的基于树突模式分析的分子间相互作用评价方法，为表征生物大分子自组装的复杂过程提供了一种快速简便的方法。
We studied the self-assembly of peptide-based ABA and CBC triblock-copolymers (obtained by bacterial expression) containing random coiled hydrophilic central B blocks flanked with helical A or C blocks. The A and C blocks were of different compositions with respect to the fraction of lysine residues which provided a higher pH-sensitivity of the copolymer solutions. The interchain interactions of the copolymer driven by external stimuli (pH and temperature) were explored in the process of macromolecular self-assembling in the thin films of the copolymer solutions deposited on the solid substrate. The interactions involved in the macromolecular association affected the morphology of the developed patterns. The polypeptide of the B block was not involved in the formation of the secondary structures, while the A and C blocks demonstrated helical folding responsible for the intermolecular association. The mechanism of the responsive behavior of the copolymers is based on the reversible assembling of the helices into coiled-coil structures upon the change of pH or temperature. It was found that at low pH values, when electrostatic repulsion was strong and the A/C blocks unfolded, assembling yielded fractal dendrites. Increasing the pH resulted in the recovery of the helical conformation of the A/C blocks and caused a transition from the fractal to compact structures. An elevation of temperature resulted in the disruption of the dendritic structures. The reported here approach to the evaluation of the intermolecular interactions, based on the analysis of the dendritic patterns, provides a rapid and simple method for the characterization of complex processes of self-assembling biomacromolecules.
登录
查看更多内容
影响因子：
8.6
作者：
WITTEN, TA;SANDER, LM
通讯作者：
SANDER, LM
影响因子：
15
作者：
Cockroft, Scott L.;Chu, John;Ghadiri, M. Reza
通讯作者：
Ghadiri, M. Reza
影响因子：
1.5
作者：
Reches, M;Gazit, E
通讯作者：
Gazit, E
影响因子：
2.9
作者：
LANGER, JS
通讯作者：
LANGER, JS
共轭聚合物和双链寡核苷酸定向分形聚集体的自组装
影响因子：
6.2
作者：
Gan, Haiyang;Li, Yuliang;Zhu, Daoben
通讯作者：
Zhu, Daoben