TRANSFORMATION OF WITHANIA-SOMNIFERA (L) DUNAL BY AGROBACTERIUM-RHIZOGENES - INFECTIVITY AND PHYTOCHEMICAL STUDIES
TRANSFORMATION OF WITHANIA-SOMNIFERA (L) DUNAL BY AGROBACTERIUM-RHIZOGENES - INFECTIVITY AND PHYTOCHEMICAL STUDIES
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DOI：
10.1002/ptr.2650080803
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发表时间：
1994-12-01
影响因子：
7.2
通讯作者：
AHUJA, PS
中科院分区：
文献类型：
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作者：
BANERJEE, S;NAQVI, AA;AHUJA, PS
以发根农杆菌A（4）、LBA 9302和LBA 9360 3个不同菌株为材料，对W.嗜睡。分析细菌的菌株特异性和转化事件的频率。不同菌株的转化能力存在显著差异。在转化能力和毛状根生长方面，A. rhizogenes菌株A（4），其次是LBA 9402，而LBA 9360菌株未能诱导转化事件。通过HPLC研究了在不同生长阶段（即4、10和24周），在A（4）诱导的毛状根系及其各自的培养基中，特别是关于醉茄素A的醉茄内酯的产生。在培养基中以及在10周龄培养物的毛状根中存在醉茄素A，突出了本研究对这种植物的商业前景的重要性。
Three different strains of Agrobacterium rhizogenes, namely A(4), LBA 9302 and LBA 9360 were used for infection to induce hairy root formation in W. somnifera. Strain specificity of the bacterium and frequency of transformation events were analysed. Significant differences were observed between the transformation ability of the different strains of Agrobacterium. The best response in terms of transformation ability and growth of the hairy roots was obtained with A. rhizogenes strain A(4), followed by LBA 9402, whereas LBA 9360 strain failed to induce a transformation event. The production of withanolides with special reference to withaferin A was studied through HPLC in the A(4) induced hairy root lines and their respective media at different growth phases (i.e. 4, 10 and 24 weeks). The presence of withaferin A in the media as well as in the hairy roots of 10-week-old cultures highlights the importance of the present study for the commercial prospects of this plant.