Substituted zinc phthalocyanine as an antimicrobial photosensitizer for periodontitis treatment
Substituted zinc phthalocyanine as an antimicrobial photosensitizer for periodontitis treatment
复制标题
替代酞菁锌作为抗菌光敏剂治疗牙周炎
DOI：
10.1142/s1088424611003276
复制
发表时间：
2011-04-01
影响因子：
1.5
通讯作者：
Huang, Mingdong
中科院分区：
文献类型：
--
作者：
Chen, Jincan;Chen, Zhuo;Huang, Mingdong
在过去的几十年中，抗生素耐药性在世界范围内的上升加剧了新抗菌剂的开发。光动力抗菌化学疗法（PACT）已成功地用于杀灭细菌。本文报道了一种新的聚赖氨酸偶联酞菁锌光敏剂（ZnPc-PL）。这种光敏剂显著灭活牙龈卟啉单胞菌，牙周炎的主要致病菌。ZnPc-PL对哺乳动物骨髓基质细胞（BMSC）和人牙周膜细胞（HPDLC）均无明显的光毒性，表明ZnPc-PL对细菌具有高度的选择性。此外，我们建立了一个实验性牙周炎模型，在比格犬体内测试抗菌效果。监测龈沟液（GCF）的量和龈沟液天冬氨酸转氨酶（AST）的活性，发现ZnPc-PL治疗组与对照组（仅激光和无治疗）相比显著降低。此外，与对照相比，具有ZnPc-PL的PACT导致细菌负荷减少100倍。综上所述，这些研究结果表明ZnPc-PL是一种很有前途的用于治疗牙周病的抗菌光敏剂。
In the last decades the worldwide rise in antibiotic resistance has intensified the development of new antimicrobial agents. Photodynamic antimicrobial chemotherapy (PACT) has been used successfully to inactivate bacteria. We herein report a new zinc phthalocyanine based photosensitizer conjugated with polylysine moiety (ZnPc-PL). This photosensitizer significantly inactivated Porphyromonas gingivalis, the primary pathogenic bacteria responsible for periodontitis. No obvious phototoxicity was found to either mammalian bone marrow stromal cells (BMSC) or human periodontal ligament cells (HPDLC), indicating the high selectivity of ZnPc-PL toward bacteria. Furthermore, we established an experimental periodontitis model on beagle dogs to test the antimicrobial efficacy in vivo. The amount of gingival crevicular fluid (GCF) and the activity of crevicular fluid aspartate aminotransferase (AST) were monitored and were found to reduce significantly in the ZnPc-PL treated group compared to the controls (laser only and no treatment). In addition, PACT with ZnPc-PL caused a reduction in the bacterial burden by 100-fold compared to controls. Taken together, these findings suggest ZnPc-PL is a promising antimicrobial photosensitizer for the treatment of periodontal diseases.