Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.
Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.
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新兴的临床微生物实验室的快速阻力测试及其对患者管理的潜在影响。
DOI：
10.1155/2014/375681
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发表时间：
2014
影响因子：
--
通讯作者：
Zautner AE
中科院分区：
文献类型：
--
作者：
Frickmann H;Masanta WO;Zautner AE
Atypical and multidrug resistance, especially ESBL and carbapenemase expressing Enterobacteriaceae, is globally spreading. Therefore, it becomes increasingly difficult to achieve therapeutic success by calculated antibiotic therapy. Consequently, rapid antibiotic resistance testing is essential. Various molecular and mass spectrometry-based approaches have been introduced in diagnostic microbiology to speed up the providing of reliable resistance data. PCR- and sequencing-based approaches are the most expensive but the most frequently applied modes of testing, suitable for the detection of resistance genes even from primary material. Next generation sequencing, based either on assessment of allelic single nucleotide polymorphisms or on the detection of nonubiquitous resistance mechanisms might allow for sequence-based bacterial resistance testing comparable to viral resistance testing on the long term. Fluorescence in situ hybridization (FISH), based on specific binding of fluorescence-labeled oligonucleotide probes, provides a less expensive molecular bridging technique. It is particularly useful for detection of resistance mechanisms based on mutations in ribosomal RNA. Approaches based on MALDI-TOF-MS, alone or in combination with molecular techniques, like PCR/electrospray ionization MS or minisequencing provide the fastest resistance results from pure colonies or even primary samples with a growing number of protocols. This review details the various approaches of rapid resistance testing, their pros and cons, and their potential use for the diagnostic laboratory.
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影响因子：
3.7
作者：
Chatterjee A;Saranath D;Bhatter P;Mistry N
通讯作者：
Mistry N
影响因子：
14.2
作者：
Bader, O.;Weig, M.;Kuhns, M.
通讯作者：
Kuhns, M.
影响因子：
3.2
作者：
Al-Zarouni, M.;Senok, A.;Panigrahi, D.
通讯作者：
Panigrahi, D.
影响因子：
9.4
作者：
Chapin, KC;Musgnug, MC
通讯作者：
Musgnug, MC
影响因子：
4.4
作者：
Caristo, Elisa;Parola, Andrea;Oderda, Giuseppina
通讯作者：
Oderda, Giuseppina