Peptide Analogues of VPP and IPP with Improved Glucose Uptake Activity in L6 Myotubes can be Released from Cereal Proteins
Peptide Analogues of VPP and IPP with Improved Glucose Uptake Activity in L6 Myotubes can be Released from Cereal Proteins
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DOI：
10.1021/acs.jafc.1c00587
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发表时间：
2021-03-01
影响因子：
6.1
通讯作者：
Wu, Jianping
中科院分区：
文献类型：
--
作者：
Liu, Ling;Zheng, Jiexia;Wu, Jianping
VPP (Val-Pro-Pro) 和 IPP (Ile-Pro-Pro) 是两种著名的抗高血压肽，可能对 2 型糖尿病 (T2DM) 有益。本研究旨在探讨VPP和IPP肽类似物对L6肌管葡萄糖摄取活性的影响。这些类似物的设计方法是将 VPP 和 IPP 的 N 端、中间或 C 端氨基酸残基每次替换为 5 个氨基酸组（极性、非极性、碱性、酸性和芳香族氨基酸）中的一个氨基酸。在测试的 26 种三肽中，IQP、IPQ、VPE 和 VEP 的葡萄糖摄取活性显着高于其亲本肽，并且均成功从大米蛋白中释放，含量分别为 5415.82 +/- 63.34、1586.77 +/- 14.94、354.07 +/- 6.56 和 596.10 +/- 2.32 ng/mg 干分别为基础，并使用多反应监测通过液相色谱-质谱 (MS)/MS 进行定量。所有四种肽均通过单磷酸腺苷激活蛋白激酶途径并伴有 4 型葡萄糖转运蛋白 (Glut4) 易位而非胰岛素信号传导途径来促进葡萄糖摄取。
VPP (Val-Pro-Pro) and IPP (Ile-Pro-Pro) are two famous antihypertensive peptides with possible benefits for type 2 diabetes mellitus (T2DM). The study was aimed to investigate the effect of peptide analogues of VPP and IPP on glucose uptake activity in L6 myotubes. The analogues were designed by replacing the N-terminal, middle, or C-terminal amino acid residues of VPP and IPP with one amino acid at a time from five amino acid groups (polar, nonpolar, basic, acidic, and aromatic amino acids). Among 26 tripeptides tested, IQP, IPQ, VPE, and VEP showed significantly higher glucose uptake activity than their parent peptides, and all were successfully released from rice proteins at the contents of 5415.82 +/- 63.34, 1586.77 +/- 14.94, 354.07 +/- 6.56, and 596.10 +/- 2.32 ng/mg dry basis, respectively, and quantified by liquid chromatography-mass spectrometry (MS)/MS using multiple reaction monitoring. All four peptides were shown to promote glucose uptake via the adenosine monophosphate-activated protein kinase pathway accompanied by glucose transporter type 4 (Glut4) translocation rather than the insulin signaling pathway.