The effect of Puccinia striiformis f. sp tritici on the levels of water-soluble carbohydrates and the photosynthetic rate in wheat leaves
The effect of Puccinia striiformis f. sp tritici on the levels of water-soluble carbohydrates and the photosynthetic rate in wheat leaves
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DOI：
10.1016/j.pmpp.2013.09.001
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发表时间：
2013-10-01
影响因子：
2.7
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
--
作者：
Chang, Qing;Liu, Jie;Kang, Zhensheng
小麦条锈病是世界范围内最严重的小麦病害。条锈菌小麦条锈病病原菌（Pst）是专性活体营养型真菌，必须通过吸器从寄主中吸收营养。最近的研究表明，糖是病原体从宿主细胞中摄取的主要营养物质。然而，糖对小麦中Pst感染的反应并不清楚。在这项研究中，水溶性碳水化合物的水平在Pst感染小麦叶片进行了调查，结果显示，除了蔗糖的所有碳水化合物略有变化。此外，蔗糖水平增加，在兼容和不兼容系统。测定了感病叶片的光合速率，发现在Pst感染的早期阶段，光合速率显著增加。此外，与光合作用相关的基因的表达谱进行了测定，通过实时PCR，发现的趋势是相同的观察到的光合速率在Pst感染小麦叶片。这些结果表明，Pst感染可以上调参与光合作用的基因，以促进光合速率，并最终诱导蔗糖积累在受感染的小麦叶片。(C)2013爱思唯尔有限公司保留所有权利。
Stripe rust is the most serious wheat disease worldwide. Puccinia striiformis f. sp. tritici (Pst), which is the causal agent of stripe rust, is an obligate biotrophic fungus and must take up nutrients from the host through its haustoria. Recent studies have indicated that sugar is the main nutrient taken up by pathogens from host cells. However, the response of sugars to Pst infection in wheat is not well known. In this study, the levels of water-soluble carbohydrates in Pst-infected wheat leaves were investigated, and the results revealed slight changes for all of the carbohydrates except sucrose. Furthermore, the sucrose levels increased in both the compatible and incompatible systems. The photosynthetic rates of infected leaves were determined and found to be significantly increased during the early stage of Pst infection. In addition, the expression profiles of genes associated with photosynthesis were assayed through real-time PCR, and the trends found were identical to that observed for the photosynthetic rate in Pst-infected wheat leaves. These findings indicate that Pst infection can upregulate genes involved in photosynthesis to promote the photosynthetic rate and ultimately induce sucrose accumulation in infected wheat leaves. (C) 2013 Elsevier Ltd. All rights reserved.