Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart
Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart
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DOI：
10.1016/j.celrep.2019.01.079
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发表时间：
2019-02-12
期刊：
影响因子：
8.8
通讯作者：
Tang, Fuchou
中科院分区：
文献类型：
--
作者：
Cui, Yueli;Zheng, Yuxuan;Tang, Fuchou
心脏是循环系统的中枢器官，心脏的正常发育对维持人类生命至关重要。在这里，我们使用单细胞RNA测序来描述来自人类胚胎的类似4000个心脏细胞的基因表达谱，并鉴定了四种主要类型的细胞：心肌细胞(CMS)、心脏成纤维细胞、内皮细胞(ECs)和瓣膜间质细胞(VICs)。房室CMS在心脏发育早期具有明显的特点。此外，CMS和成纤维细胞都显示出基因表达的逐步变化。随着发育的进行，血管内皮细胞可能参与重塑阶段，内皮细胞表现出特定位置的特征。最后，我们比较了人类和小鼠之间的基因表达谱，并确定了人类心脏发育的一系列独特特征。我们的研究为阐明活体人类心脏发育的机制奠定了基础，并为理解心脏再生提供了潜在的线索。
The heart is the central organ of the circulatory system, and its proper development is vital for maintaining human life. Here, we used single-cell RNA sequencing to profile the gene expression landscapes of similar to 4,000 cardiac cells from human embryos and identified four major types of cells: cardiomyocytes (CMs), cardiac fibroblasts, endothelial cells (ECs), and valvar interstitial cells (VICs). Atrial and ventricular CMs acquired distinct features early in heart development. Furthermore, both CMs and fibro-blasts show stepwise changes in gene expression. As development proceeds, VICs may be involved in the remodeling phase, and ECs display location-specific characteristics. Finally, we compared gene expression profiles between humans and mice and identified a series of unique features of human heart development. Our study lays the groundwork for elucidating the mechanisms of in vivo human cardiac development and provides potential clues to understand cardiac regeneration.