ERYTHROPOIETIN IN POLYCYSTIC KIDNEYS
ERYTHROPOIETIN IN POLYCYSTIC KIDNEYS
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DOI：
10.1172/jci114280
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发表时间：
1989-10-01
影响因子：
15.9
通讯作者：
KURTZ, A
中科院分区：
文献类型：
--
作者：
ECKARDT, KU;MOLLMANN, M;KURTZ, A
对18例常染色体显性遗传性多囊肾病（ADPKD）患者肾组织促红细胞生成素（EPO）的形成进行了研究。在12例血液透析患者和6例终末前肾衰竭患者血清中，EPO为29 ± 0.01。7和16 .+-。1.5 mU/ml，血红蛋白浓度为11.0 ± 0.001。0.6和12.7 .+-。1.2 g/dl。通过体内抽吸或肾切除术后立即获得来自总共357个肾囊肿的囊液。囊肿含有不同浓度的生物活性EPO，从不可检测的值高达3.2 U/ml。在钠浓度> 100 mmol/L的囊肿中观察到EPO的显著富集，表明与近端肾小管畸形相关。囊肿中的EPO浓度与蛋白质浓度和囊液的氧分压均不相关。使用人EPO的cDNA探针，通过原位杂交技术将EPO的mRNA定位于囊壁的基质细胞中。我们的研究结果表明，单个间质细胞并列近端肾小管囊肿可能产生EPO独立的氧气压力内的囊肿，这改善了贫血在终末期多囊肾疾病。
Erythropoietin (EPO) formation in kidneys of 18 patients with autosomal dominant polycystic kidney disease (ADPKD) was investigated. In 12 patients on hemodialysis and in 6 patients with preterminal renal failure serum, EPO was 29 .+-. 7 and 16 .+-. 1.5 mU/ml and hemoglobin concentrations were 11.0 .+-. 0.6 and 12.7 .+-. 1.2 g/dl, respectively. Cyst fluid from a total of 357 renal cysts was obtained by either in vivo aspiration or immediately after nephrectomy. The cysts contained variable concentrations of bioactive EPO from undectable values up to 3.2 U/ml. A pronounced enrichment of EPO was observed in cysts with sodium concentration > 100 mmol/liter, suggesting an association with proximal tubular malformations. The EPO concentrations in the cysts were neither correlated with the protein concentration nor with the oxygen pressure of the cyst fluid. Using a cDNA probe for human EPO, mRNA for EPO was localized in stroma cells of the cyst walls by an in situ hybridization technique. Our findings suggest that single interstitial cells juxtaposed to proximal tubular cysts may produce EPO independent of the oxygen pressure inside the cysts, which ameliorates the anemia during end-stage polycystic kidney disease.