IDENTIFICATION OF S71-RELATED HUMAN ENDOGENOUS RETROVIRAL SEQUENCES WITH FULL-LENGTH POL GENES
IDENTIFICATION OF S71-RELATED HUMAN ENDOGENOUS RETROVIRAL SEQUENCES WITH FULL-LENGTH POL GENES
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DOI：
10.1006/viro.1995.1287
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发表时间：
1995-06-01
期刊：
影响因子：
3.7
通讯作者：
LEIBMOSCH, C
中科院分区：
文献类型：
--
作者：
HALTMEIER, M;SEIFARTH, W;LEIBMOSCH, C
人类基因组包含与类人猿肉瘤相关病毒SSAV相关的序列。其中一种内源性逆转录病毒元件S71在pol基因中被截断，并携带一个单独的HERV-K LTR插入。利用PCR方法，我们现在已经确定了更多的S71相关逆转录病毒元件，这些元件缺乏HERV-K LTR插入，并包含一个全长逆转录病毒逆转录酶。其中两个序列，pCRTK1和pCRTK6，被克隆并进一步表征。克隆pCRTK1和pCRTK6在pol基因的tether区域与S71核苷酸同源性在85 - 90%之间，这表明pCRTK1和pCRTK6显然属于c型相关的人类内源性逆转录病毒元件的S71亚群。一些灭活逆转录酶的点突变在pCRTK1和pCRTK6中位于相同的位置。因此，我们假设这些与s71相关的元件通过重新整合成为有缺陷的原病毒而分散在人类基因组中，可能是利用其他反转录转座子或细胞基因在反转录中提供的反转录转座酶。对猿和旧大陆猴中S71相关元件的检测显示，在插入HERV-K LTR之前，灵长类谱系中S71逆转录酶序列的缺失已经发生。(C) 1995学术出版社，Inc。
The human genome contains sequences related to the simian sarcoma-associated virus SSAV. One of these endogenous retroviral elements, S71, is truncated in the pol gene and carries an insertion of a solitary HERV-K LTR. Using a PCR approach we have now identified further S71-related retroviral elements that lack the HERV-K LTR insertion and contain a full-length retroviral reverse transcriptase. Two of these sequences, pCRTK1 and pCRTK6, were cloned and further characterized. Clones pCRTK1 and pCRTK6 showed between 85 and 90% nucleotide homology to each other and to S71 within the ''tether'' region of the pol gene, indicating that pCRTK1 and pCRTK6 clearly belong to the S71 subgroup of C-type-related human endogenous retroviral elements. Some point mutations inactivating the reverse transcriptase are located at the same positions in pCRTK1 and pCRTK6. Therefore, we assume that these S71-related elements were dispersed in the human genome by reintegration as defective proviruses, probably using enzymes for retrotransposition provided in trans by other retrotransposons or by cellular genes. Examination of the presence of S71-related elements in apes and Old World monkeys revealed that the deletion of reverse transcriptase sequences in S71 has occurred in the lineage of primates prior to the insertion of the HERV-K LTR. (C) 1995 Academic Press, Inc.