Layer-by-layer assembly sensitive electrochemical sensor for selectively probing L-histidine based on molecular imprinting sol-gel at functionalized indium tin oxide electrode.
Layer-by-layer assembly sensitive electrochemical sensor for selectively probing L-histidine based on molecular imprinting sol-gel at functionalized indium tin oxide electrode.
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DOI：
10.1016/j.bios.2010.06.062
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发表时间：
2010-10
影响因子：
12.6
通讯作者：
Zhaohui Zhang;Yufang Hu;Huabin Zhang;Lijuan Luo;S. Yao
中科院分区：
文献类型：
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作者：
Zhaohui Zhang;Yufang Hu;Huabin Zhang;Lijuan Luo;S. Yao
将分子印迹膜与多壁碳纳米管（MWNTs）相结合，成功构建了一种新型的L-组氨酸分子印迹电化学传感器。该传感器是通过逐步修饰的多壁碳纳米管和分子印迹聚合物（MIPs）薄膜，通过溶胶-凝胶技术的氧化铟锡（ITO）电极上制作的。引入的MWNTs表现出显着的增强的敏感性的MIP传感器。同时，该分子印迹膜对目标分子l-组氨酸具有高灵敏度和良好的选择性。采用扫描电镜（SEM）、循环伏安法（CV）、微分脉冲伏安法（DPV）和电流i-t曲线等电化学方法对印迹传感器进行表征。L-组氨酸的线性范围为2.0μmolL-1 ~ 1.0mmolL-1，检出限为5.8×10 - 9 molL-1。该印迹电化学传感器成功地应用于人血清中l-组氨酸的检测。
A novel sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of l-histidine by combination of a molecular imprinting film and multi-walled carbon nanotubes (MWNTs). The sensor was fabricated onto an indium tin oxide (ITO) electrode via stepwise modification of MWNTs and a thin film of molecularly imprinted polymers (MIPs) via sol–gel technology. The introduced MWNTs exhibited noticeable enhancement on the sensitivity of the MIPs sensor. Meanwhile, the molecularly imprinted film displayed high sensitivity and excellent selectivity for the target molecule l-histidine. The proposed imprinted sensor was characterized by using scanning electron microscope (SEM) and electrochemical methods involving cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometric i–t curve. A linear ranging from 2.0μmolL−1to 1.0mmolL−1for the detection of l-histidine was observed with the detection limit of 5.8×10−9molL−1for S/N=3. This imprinted electrochemical sensor was successfully employed to detect l-histidine in human blood serum.