Inhibition of HYBID (KIAA1199)-mediated hyaluronan degradation and anti-wrinkle effect of Geranium thunbergii extract
Inhibition of HYBID (KIAA1199)-mediated hyaluronan degradation and anti-wrinkle effect of Geranium thunbergii extract
复制标题
天竺葵提取物抑制 HYBID (KIAA1199) 介导的透明质酸降解和抗皱作用
DOI：
10.1111/jocd.12813
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发表时间：
2018
影响因子：
2.3
通讯作者：
Takahashi Yoshito
中科院分区：
文献类型：
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作者：
Yoshida Hiroyuki;Yamazaki Kohei;Komiya Aya;Aoki Mika;Nakamura Tomomi;Kasamatsu Shinya;Murata Takeshi;Sayo Tetsuya;Okada Yasunori;Takahashi Yoshito
背景透明质酸（HA）是皮肤细胞外基质的重要组成部分。HA减少在真皮和过度表达的HYBID（KIAA 1199），HA降解的关键分子在皮肤成纤维细胞，是牵连在面部skin allowing.AimsWe旨在获得抗皱剂（S）通过筛选抑制HYBID介导的HA degradation.MethodsVarious植物提取物筛选抑制HA降解HYBID稳定转染HEK 293（HYBID/HEK 293）。在皮肤成纤维细胞和HYBID/HEK 293细胞中研究了老鹳草提取物对HA降解活性和HYBID mRNA和蛋白表达的抑制作用。通过在皮肤成纤维细胞中制备代谢性放射性标记的HA来评价新产生的HA的尺寸分布。在健康的日本女性（n = 21）中进行了一项双盲、随机和安慰剂对照的研究，通过用G. Thunbergi提取物或安慰剂配制的洗剂局部治疗面部的每一侧8周。只有G. thunbergi提取物能消除皮肤成纤维细胞中的HA解聚，HYBID/通过下调HYBID mRNA和蛋白质表达以及抑制HYBID介导的HA降解活性，在HEK 293细胞中表达。尽管未处理的皮肤成纤维细胞产生多分散的HA，但经G. thunbergiiextract处理的细胞产生高分子量HA。治疗与G. thunbergiiextraction配方洗剂显着改善皮肤弹性和减少皮肤皱纹分数在外眼角相比，安慰剂formulation. ConclusionsGerumthunbergiiextraction抑制HYBID介导的HA降解在体外，并显示抗皱活性在体内伴随着皮肤弹性的改善。我们的研究通过抑制HYBID介导的HA降解为抗皱护理提供了一种可能的策略。
BackgroundHyaluronan (HA) is an essential constituent of extracellular matrix in the skin. HA reduction in the dermis and overexpression of HYBID (KIAA1199), a key molecule for HA degradation in skin fibroblasts, are implicated in facial skin wrinkling.AimsWe aimed to obtain anti‐wrinkle agent(s) by screening for inhibition of HYBID‐mediated HA degradation.MethodsVarious plant extracts were screened for inhibition of HA degradation in HYBID‐stable transfectants in HEK293 (HYBID/HEK293). Inhibition of HA‐degrading activity and HYBID mRNA and protein expression byGeranium thunbergiiextract was studied in skin fibroblasts and HYBID/HEK293 cells. Size distribution of newly produced HA was evaluated by preparing metabolically radiolabeled HA in skin fibroblasts. A double‐blind, randomized, and placebo‐controlled study was performed in healthy Japanese women (n = 21) by topically treating each side of the face with a lotion formulated withG. thunbergiiextract or placebo for 8 weeks.ResultsAmong the plant extracts tested, onlyG. thunbergiiextract abolished HA depolymerization in skin fibroblasts and HYBID/HEK293 cells by down‐regulating HYBID mRNA and protein expression and by inhibiting HYBID‐mediated HA‐degrading activity. Although untreated skin fibroblasts produced polydispersed HA,G. thunbergiiextract‐treated cells produced high‐molecular‐weight HA. Treatment withG. thunbergiiextract‐formulated lotion significantly improved skin elasticity and reduced skin wrinkling scores at the outer eye corner compared with the placebo formulation.ConclusionsGeranium thunbergiiextract inhibited HYBID‐mediated HA degradation in vitro and showed anti‐wrinkle activity in vivo accompanying the improvement in skin elasticity. Our study provides a possible strategy for anti‐wrinkle care through inhibition of HYBID‐mediated HA degradation.