Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality
Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality
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DOI：
10.1093/humrep/17.6.1591
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发表时间：
2002-06-01
影响因子：
6.1
通讯作者：
Haines, CJ
中科院分区：
文献类型：
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作者：
Chiu, TTY;Rogers, MS;Haines, CJ
背景：卵泡微环境是卵母细胞发育的重要决定因素。本研究的目的是探讨人卵泡液（FF）中肌醇（MI）含量是否与较好的卵母细胞质量有关。方法：共招募53例接受体外受精治疗的患者进行前瞻性观察研究。将采集的FF和血清样本分为两组：A组为与成熟和受精卵母细胞相关的FF， B组为来自未成熟和未受精卵母细胞的卵泡。结果：两组患者年龄、使用HMG总安瓶数、刺激天数、FSH基础水平、HCG当日雌二醇（E-2）水平、血清心肌梗死（MI）含量无显著差异。A组FF体积和心肌梗死含量显著高于B组（P < 0.05）。FF中MI水平与相应FF样品中E-2含量呈正相关，也与胚胎质量相关。结论：我们认为，人FF中较高浓度的MI和E-2似乎在卵泡成熟中发挥作用，并提供了优质卵母细胞的标志。
BACKGROUND: The follicular microenvironment is an important determinant of oocyte development. The aim of this study was to examine whether the myo-inositol (MI) content in human follicular fluid (FF) was associated with better oocyte quality. METHODS: A total of 53 patients treated with IVF was recruited to a prospective observational study. FF and serum samples collected were divided into two groups: group A consisted of FF associated with matured and fertilized oocytes, whilst group B was from follicles with immature and unfertilized oocytes. RESULTS: Patient's age, total ampoules of HMG used, days of stimulation, basal levels of FSH, estradiol (E-2) levels on the day of HCG, and serum MI content were not significantly different between the two groups. FF volume and its MI content were significantly higher in group A compared with group B (P < 0.05). The levels of MI in FF were positively correlated with the amount of E-2 in their corresponding FF samples and also correlated with embryo quality. CONCLUSIONS: We propose that higher concentrations of MI and E-2 in human FF appear to play a role in follicular maturity and provide a marker of good quality oocytes.