Natural killer (NK) cells prevent virus production in cell culture
Natural killer (NK) cells prevent virus production in cell culture
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DOI：
10.1038/sj.bmt.1701825
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发表时间：
1999-07-01
影响因子：
4.8
通讯作者：
Nagler, A
中科院分区：
文献类型：
--
作者：
Baraz, L;Khazanov, E;Nagler, A
自然杀伤（NK）细胞(CD3(-)/CD16（+)/CD56（+）淋巴细胞）在早期免疫防御病毒感染中发挥重要作用，这一事实对骨髓移植后严重免疫抑制的患者具有重要意义。在这项研究中，我们证明NK细胞优先溶解感染单纯疱疹病毒1型（HSV-1）和牛痘病毒（VV）的人结肠腺癌（Colo-205）肿瘤细胞和感染VV的自体T细胞。采用病毒感染中心（IC）法对这一现象进行了评估，并与标准cr -51释放试验结果进行了比较。使用IC试验，我们发现NK细胞在感染的早期阶段裂解病毒感染细胞，从而阻止病毒向邻近细胞传播，通过碘化丙啶（PI）渗透验证的cr -51释放试验表明NK介导的抗病毒活性的早期作用不是膜损伤的结果。NK细胞对HSV-1感染的Colo-205细胞的作用似乎与主要组织相容性复合体（MHC） I类分子的表达水平无关，而杀死自体vv -1感染的T细胞与MHC I类表达的减少相关。我们的研究结果表明，除了MHC之外，还有其他因素在NK细胞介导的病毒感染细胞的裂解中发挥作用。这可能对骨髓移植后免疫抑制严重的患者具有重要的临床意义。
Natural killer (NK) cells (CD3(-)/CD16(+)/CD56(+) lymphocytes) play an important role in early immune defense against viral infection, a fact which is of prime significance for heavily immunosuppressed patients after bone marrow transplantation. In this study we demonstrate that NK cells preferentially lyse human colon adenocarcinoma (Colo-205) tumor cells infected with herpes simplex virus type 1 (HSV-1) and vaccinia virus (VV) and autologous T cells infected with VV. This phenomenon was assessed by the viral infectious center (IC) method and compared with the results obtained by means of the standard Cr-51-release assay, Using the IC assay,we found that NK cells lyse virus infected cells at an early stage of infection, thereby preventing viral dissemination to neighboring cells, Cr-51-release assay verified by propidium iodide (PI) penetration showed that the early effects of NK mediated anti-viral activity are not the result of membrane damage. The effect of NK cells on HSV-1 infected Colo-205 cells appears to be independent of the level of expression of major histocompatibility complex (MHC) class I molecules while the killing of autologous VV-infected T cells correlates with a reduction in MHC class I expression. Our results suggest that additional factors besides MHC play a role in the regulation of NK cell-mediated lysis of virus infected cells. This may be of clinical importance in patients who are heavily immunosuppressed after bone marrow transplantation.