NEUTROPHIL ACTIN DYSFUNCTION AND ABNORMAL NEUTROPHIL BEHAVIOR
NEUTROPHIL ACTIN DYSFUNCTION AND ABNORMAL NEUTROPHIL BEHAVIOR
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DOI：
10.1056/nejm197411212912101
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发表时间：
1974-01-01
影响因子：
158.5
通讯作者：
STOSSEL, TP
中科院分区：
文献类型：
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作者：
BOXER, LA;HEDLEYWH.T;STOSSEL, TP
一名婴儿反复出现细菌感染，但未能产生脓。他的中性粒细胞移动缓慢，以正常速度的15%摄取颗粒。颗粒内容物分泌到吞噬体是2.5倍以上的控制校正受损的摄入。由于中性粒细胞异常的肌动蛋白的作用进行了研究。与正常人相比，患者的中性粒细胞中可见较少的富含微生物活性物质的伪足。在正常和病人的甘油中性粒细胞中，重的解肌球蛋白与微丝结合，表明微丝是肌动蛋白聚合物。患者和正常的中性粒细胞含有等量的肌动蛋白（13.8%和14.3%）。然而，在用氯化钾聚合治疗后，患者的肌动蛋白沉淀量减少了七倍。在中性粒细胞提取物中的可聚合性差的肌动蛋白的协会表现出异常的运动，摄取和脱粒是这种蛋白参与这些功能的证据。（《新英格兰医学杂志》291：1093-1099，1974）
An infant had recurrent bacterial infections but failed to produce pus. His neutrophils migrated slowly and ingested particles at 15 per cent of the rate of normal. The secretion of granule contents into phagosomes was 2.5 times greater than the control when corrected for the impaired ingestion.Because of the neutrophil abnormalities the role of actin was studied. As compared with normal, fewer microfilament-rich pseudopodia were visible in the patient's neutrophils. Heavy meromyosin bound to microfilaments in both normal and the patient's glycerinated neutrophils, indicating that the filaments were actin polymers. The patient's and normal neutrophils contained equal quantities (13.8 and 14.3 per cent) of actin protein. However, seven times less of the patient's actin sedimented after polymerizing treatment with potassium chloride. The association of a poorly polymerizable actin in extracts of neutrophils exhibiting abnormal locomotion, ingestion and degranulation is evidence for the participation of this protein in these functions. (N Engl J Med 291: 1093–1099, 1974)