Morphological and topological transformation of membrane vesicles
Morphological and topological transformation of membrane vesicles
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DOI：
10.1023/a:1019971429702
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发表时间：
2002-01-01
影响因子：
1.8
通讯作者：
Hotani, H
中科院分区：
文献类型：
--
作者：
Nomura, F;Honda, M;Hotani, H
脂质体是由脂质双层膜组成的微区室，其特征与生物膜非常相似。为了形成人工细胞样结构，我们产生了含有细胞骨架亚基蛋白质（微管蛋白或肌动蛋白）的脂质体。球形脂质体被转化为双极或细胞样的形状由微管的封装亚基聚合产生的机械力。圆盘状或哑铃状脂质体是通过聚合包裹的肌动蛋白而开发的。高强度暗视野光学显微镜观察到脂质体形态变化的动态过程，如膜囊泡的融合和分裂等拓扑结构变化在细胞活动中起着重要作用。为了研究这些过程的机制，我们在真实的时间可视化的脂质体进行拓扑变换。发现了多种新颖的拓扑结构变化，包括脂质体的开放和内囊泡的直接排出。
Liposomes are micro-compartments made of lipid bilayer membranes with characteristics quite similar to those of biological membranes. To form artificial cell-like structures, we generated liposomes that contained subunit proteins of cytoskeletons: tubulin or actin. Spherical liposomes were transformed into bipolar or cell-like shapes by mechanical forces generated by the polymerization of encapsulated subunits of microtubules. Disk- or dumbbell-shaped liposomes were developed by the polymerization of encapsulated actin. Dynamic processes of morphological transformations of liposomes were visualized by high intensity dark-field light microscopy.Topological changes, such as fusion and division of membrane vesicles, play an essential role in cellular activities. To investigate the mechanism of these processes, we visualized in real time the liposomes undergoing topological transformation. A variety of novel topological transformations were found, including the opening-up of liposomes and the direct expulsion of inner vesicles.