STUDIES ON MECHANISM OF ACTION OF NALIDIXIC-ACID
STUDIES ON MECHANISM OF ACTION OF NALIDIXIC-ACID
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DOI：
10.1128/aac.4.4.479
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发表时间：
1973-01-01
影响因子：
4.9
通讯作者：
STERNGLA.R
中科院分区：
文献类型：
--
作者：
BOURGUIG.GJ;LEVITT, M;STERNGLA.R
通过三种独立的技术(吸收分光光度法、脱氧核糖核酸[DNA]熔融温度的测量和平衡透析)，还没有发现Nalidixic与纯化的DNA结合的证据。此外，还没有发现证据支持纳利地酸被细菌细胞永久修饰为一种新的活性化合物的假设。通过使用Bonhoeffer及其同事开发的体外DNA复制系统，已证明从对奈利地酸敏感的细胞中提取的可溶性提取物可使耐奈利地酸的细胞裂解产物的DNA合成对奈利地酸敏感。提取物中的活性只存在于敏感细胞中，不能透析性和热敏性。最后，对已知的两个已知的大肠杆菌耐甲苯二甲酸突变体进行了测试，以确定它们是否为运输突变体。已证明NAL Br1是运输突变体，而NAL Ar1不是。
With three independent techniques (absorption spectrophotometry, measurement of the deoxyribonucleic acid [DNA] melting temperature, and equilibrium dialysis), no evidence has been found for the binding of nalidixic acid to purified DNA. Also, no evidence has been found to support the hypothesis that nalidixic acid is permanently modified to a new, active compound by the bacterial cell. By using an in vitro DNA replication system developed by Bonhoeffer and colleagues, soluble extracts from nalidixic acid-sensitive cells have been shown to confer nalidixic acid sensitivity on the DNA synthesis of lysates from nalidixic acid-resistant cells. The activity in the extracts is only present in sensitive cells and is nondialyzable and heat sensitive. Finally, two known nalidixic acid-resistant mutants ofEscherichia coli, mapping atnal Aandnal B, respectively, have been tested to determine whether either of them is a transport mutant. It has been shown thatnal Bris a transport mutant whereasnal Aris not.