Activated charcoal significantly reduces the amount of colchicine released from Gloriosa superba in simulated gastric and intestinal media
Activated charcoal significantly reduces the amount of colchicine released from Gloriosa superba in simulated gastric and intestinal media
复制标题
活性炭显着减少模拟胃和肠道介质中嘉兰菊释放的秋水仙碱量
作者：
Shukry Zawahir;I. Gawarammana;P. Dargan;Mahfoudh Abdulghni;A. Dawson
摘要背景：在斯里兰卡，用含有秋水仙素的植物Gloriosa Superba中毒是很常见的。目的：测定秋水仙碱在模拟胃肠道介质中5 g的释放量，并考察活性碳(AC)对秋水仙碱的结合效果。方法：采用USP-II溶出仪制备样品，采用高效液相色谱法测定秋水仙碱的含量。结果：120 时，秋水仙碱在胃液中的累积释放量(2883 μg/g)显著高于肠液(1015 μg/g)(p <； .001)。秋水仙碱在胃液中的平均累积浓度为2883.15 ± 1295.63、578.25 ± 366.26和345.60 ± 200.08 μg/g；在肠道介质中的释放量分别为1014.75 ± 268.16、347.40 ± 262.61和251.55 ± 285.72 μg/g。在两种培养基中加入50 g的AC使秋水仙碱不能被检测到(<；0.1 μg/ml)。结论：块茎中秋水仙碱的释放量最高。秋水仙碱的释放和达到饱和、平衡溶出所需的时间主要取决于植物部分的物理化学性质。秋水仙碱在体外与AC的显著结合表明，AC在服用Gloriosa Superba后到医院就诊的患者中具有净化作用。
Abstract Background: Poisoning with Gloriosa superba, a plant containing colchicine, is common in Sri Lanka. Objectives: This study was to estimate release of colchicine from 5 g of different parts of Gloriosa superba in simulated gastric and intestinal media, and examine the binding efficacy of activated charcoal (AC) to colchicine within this model. Methods: A USP dissolution apparatus-II was used to prepare samples for analysis of colchicine using HPLC. Results: Cumulative colchicine release from tuber in gastric media at 120 minutes was significantly higher (2883 μg/g) than in intestinal media (1015 μg/g) (p < .001). Mean ± SD cumulative colchicine concentration over 2 hours from tuber, leaves and trunk in gastric medium was 2883.15 ± 1295.63, 578.25 ± 366.26 and 345.60 ± 200.08 μg/g respectively and the release in intestinal media was 1014.75 ± 268.16, 347.40 ± 262.61 and 251.55 ± 285.72 μg/g respectively. Introduction of 50 g of AC into both media made colchicine undetectable (<0.1 μg/ml). Conclusions: The tuber released the highest quantity of colchicine. The colchicine release and elapse time to achieve saturated, equilibrium dissolution mainly depends on physicochemical properties of plant part. Significant in vitro binding of colchicine to AC suggests that AC has a role in decontamination of patients presenting to hospital after ingestion of Gloriosa superba.