Chlamydia pneumoniae DNA in patients with symptomatic carotid atherosclerotic disease.
Chlamydia pneumoniae DNA in patients with symptomatic carotid atherosclerotic disease.
复制标题
有症状的颈动脉粥样硬化性疾病患者的肺炎衣原体 DNA。
DOI：
10.1067/mva.2003.200
复制
发表时间：
2003
影响因子：
4.3
通讯作者：
M. del Piano
中科院分区：
文献类型：
--
作者：
R. Sessa;M. Di Pietro;G. Schiavoni;I. Santino;F. Benedetti;R. Perna;S. Romano;M. del Piano
客观化
我们评估了动脉粥样硬化性颈动脉斑块、淋巴结和外周血单核细胞(PBMC)中肺炎衣原体的存在与症状性颈动脉粥样硬化性疾病的相关性。
方法
51名连续接受颈动脉内膜切除术的患者入选；其中18名患者有症状性疾病，33名患者无症状疾病。用聚合酶链式反应(PCR)检测动脉粥样硬化性颈动脉斑块、淋巴结和外周血单核细胞中肺炎衣原体DNA。用微量免疫荧光试验检测肺炎衣原体抗体。
结果
有症状性疾病患者颈动脉斑块中肺炎衣原体DNA阳性率为44.4%，无症状性疾病患者为30.3%(P=0.48)。有症状组和无症状组的淋巴组织中c肺炎DNA的阳性率分别为33.3%和18.2%(P=0.19)。有症状性疾病患者PBMC中肺炎衣原体DNA的阳性率(72.2%)明显高于无症状疾病患者(30.3%)(P=0.01)。血清学结果支持肺炎衣原体DNA与症状性颈动脉粥样硬化性疾病的关系，因为在有症状性疾病的患者中，抗衣原体免疫球蛋白(Ig)A抗体的血清阳性率显著高于无症状性疾病患者(P=0.02)。
结论
PBMC中肺炎衣原体的检测表明肺炎衣原体可能与症状性颈动脉粥样硬化性疾病有关。尽管我们认为有必要对聚合酶链式反应方法进行标准化，并评估这些检测的敏感性、特异性和预测值，但我们认为，外周血单核细胞中肺炎衣原体DNA的存在是血管内衣原体感染风险的有效替代标志物。只有长期的队列研究和干预性试验才能阐明肺炎衣原体的致病作用。
OBJECTIVE
We evaluated the correlation between the presence of Chlamydia pneumoniae in atherosclerotic carotid plaques, lymph nodes, and peripheral blood mononuclear cells (PBMCs), and symptomatic carotid atherosclerotic disease.
METHODS
Fifty-one consecutive patients undergoing carotid endarterectomy were enrolled; 18 of the 51 patients had symptomatic disease, whereas 33 had asymptomatic disease. Detection of C pneumoniae DNA in atherosclerotic carotid plaques, lymph nodes, and PBMCs was performed with polymerase chain reaction (PCR). C pneumoniae antibodies were measured with a microimmunofluorescence test.
RESULTS
C pneumoniae DNA in atherosclerotic carotid plaques was detected in 44.4% of patients with symptomatic disease and in 30.3% of those with asymptomatic disease (P =.48). C pneumoniae DNA in lymph nodes was detected in 33.3% of patients with symptomatic disease and 18.2% of those with asymptomatic disease (P =.19). Prevalence of C pneumoniae DNA in PBMCs was significantly higher in patients with symptomatic disease (72.2%) compared with those with asymptomatic disease (30.3%) (P =.01). Serologic results support the association between C pneumoniae DNA in PBMCs and symptomatic carotid atherosclerotic disease, because seropositivity for anti-chlamydial immunoglobulin (Ig) A antibodies was significantly higher in PCR-positive PBMCs in patients with symptomatic disease compared with patients with asymptomatic disease (P =.02).
CONCLUSIONS
Detection of C pneumoniae in PBMCs shows that C pneumoniae may be associated with symptomatic carotid atherosclerotic disease. Although we believe there is need for standardization of PCR methods and for assessing sensitivity, specificity, and predictive values of the tests, we suggest that presence of C pneumoniae DNA in PBMCs is a valid surrogate marker of risk for endovascular chlamydial infection. Only long-term cohort studies and interventional trials will clarify the etiopathogenic role of C pneumoniae.
心血管动脉粥样硬化中肺炎衣原体检测的特异性：无辜旁观者假设的评估。
DOI：
--
发表时间：
1997
期刊：
The American journal of pathology.
影响因子：
--
作者：
Jackson,LA;Campbell,LA;Schmidt,RA;Kuo,CC;Cappuccio,AL;Lee,MJ;Grayston,JT
通讯作者：
Grayston,JT