Temporal regulation of gene expression in the blastoderm Drosophila embryo.
Temporal regulation of gene expression in the blastoderm Drosophila embryo.
复制标题
果蝇胚胚层基因表达的时间调控。
DOI：
10.1101/gad.5.10.1800
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发表时间：
1991
影响因子：
10.5
通讯作者：
Schubiger,G
中科院分区：
文献类型：
--
作者：
Yasuda,GK;Baker,J;Schubiger,G
果蝇胚胎在第14周期经历发育转变，启动异步化有丝分裂周期，并显着提高合子转录速率。核质比被认为是在时间上调节这种发育转变的单一因素。我们改变了胚胎中的比例，并分析了对细胞周期程序和特定基因转录本的影响。之所以选择这些基因，是因为它们的转录本在第14周期中通常会经历模式的变化。我们发现有证据表明，核质比在局部被读取和解释，以调节细胞周期程序。根据转录产物对比例变化的反应，我们发现有证据表明，至少有两类时间调控机制控制着这些转录产物。因此，我们提出了两类相应的转录本：(1)核质比相关；(2)核质比无关或时间相关。不依赖比例的转录本的时间调节可能依赖于发育时间。我们得出结论，多种时间调节模式是果蝇胚胎发生发育转变事件的基础。
The Drosophila embryo undergoes a developmental transition during cycle 14 when it initiates asynchronous mitotic cycles and markedly increases its rate of zygotic transcription. The nucleo-cytoplasmic ratio has been proposed to be the single factor that temporally regulates this developmental transition. We altered the ratio in the embryo and analyzed the consequences on the cell cycle program and on the transcripts of specific genes. These genes were chosen because their transcripts normally undergo changes in pattern during cycle 14. We found evidence that the nucleo-cytoplasmic ratio is read and interpreted locally to regulate the cell cycle program. Based on the response of the transcripts to changes in the ratio, we found evidence that at least two classes of temporal regulatory mechanisms control these transcripts. We therefore propose two corresponding classes of transcripts: (1) nucleo-cytoplasmic ratio dependent; and (2) nucleo-cytoplasmic ratio independent or time correlated. The temporal regulation of the ratio-independent transcripts may be dependent on developmental time. We conclude that multiple modes of temporal regulation underlie the events of the developmental transition in Drosophila embryogenesis.