Detection of a 5′ end subgenome of hepatitis C virus terminating at nucleotide 384 in patients' plasma and liver tissues
Detection of a 5′ end subgenome of hepatitis C virus terminating at nucleotide 384 in patients' plasma and liver tissues
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DOI：
10.1111/j.1365-2893.2006.00745.x
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发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Yoshikura, H.
中科院分区：
文献类型：
--
作者：
Shimizu, Y. K.;Hijikata, M.;Yoshikura, H.
Quadri和Negro [Dig Liver Dis 2001; 33：480]报道了在肝移植后复发性丙型肝炎患者的肝脏中，丙型肝炎病毒（HCV）的5'末端基因组RNA的分布大于其3'末端。我们不仅通过使用稀释和实时聚合酶链反应方法定量各种标本中的5'端亚基因组来证实他们的结果，而且还发现这些亚基因组终止于病毒基因组的核苷酸（nt）384或其紧邻的上游。除了少数例外，血浆中的亚基因组一致地终止于该位置，而肝脏中的亚基因组在nt 384上游的各个点处更不均匀。未检测到终止于nt 384下游某些点的亚基因组群体。5'端亚基因组的量在患者的临床过程中波动，但超过了包括完整基因组的较长尺寸的HCV基因组的量，并且当5'端亚基因组的相对比例增加时，较长尺寸的HCV RNA的量趋于减少，这表明5'端亚基因组对病毒复制的抑制作用。
Quadri and Negro [Dig Liver Dis 2001; 33: 480] reported greater distribution of 5' end genomic RNA of hepatitis C virus (HCV) over its 3' end in the liver of patients with recurrent hepatitis C after liver transplantation. We not only confirmed their results by quantifying the 5' end subgenomes in various specimens by using dilution and real-time polymerase chain reaction methods, but also discovered that such subgenomes terminated at nucleotide (nt) 384 of the viral genome or in its immediate upstream. The subgenomes in the plasma uniformly, with a few exceptions, ended at this position, while those in the liver more heterogeneously at various points upstream of nt 384. Subgenome populations ending some points in the downstream of nt 384 were not detected. The amount of the 5' end subgenome, while fluctuating during the clinical course of the patients, exceeded that of the longer sized HCV genomes which included the intact genome, and, when the relative ratio of the 5' end subgenome increased, the amount of longer sized HCV RNA tended to decrease, suggesting a suppressive effect of the 5' end subgenome on viral replication.