Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis.
Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis.
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中内胚层规格取决于斑马鱼胚胎发生过程中 V 类 POU 型转录因子 Pou2 的功能。
DOI：
10.1111/j.1440-169x.2012.01369.x
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Yamasu K.
中科院分区：
文献类型：
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作者：
Khan A;Nakamoto A;Tai M;Saito S;Nakayama Y;Kawamura A;Takeda H;Yamasu K.
Zebrafishpou 2编码V类POU转录因子，与小鼠Oct-3/4同源，通过分析pou 2突变胚胎，发现其与发育的不同方面有关，如背腹图案形成、内胚层形成和脑区域化。在本研究中，我们首先通过mRNA注射进行pou 2修饰基因的过表达，发现pou 2及其活性形式（vp-pou 2）增强中胚层形成并抑制内胚层特化，而抑制性pou 2（en-pou 2）以不同的方式影响中胚层和内胚层的形成。为了避免在发育过程中不同pou 2功能可能引起的并发症，我们使用了一个含有可诱导的pou 2（HEP）的转基因系，它可以以显性负性方式发挥作用。我们发现，在囊胚中期通过HEP诱导抑制内源性spou 2以牺牲中胚层为代价增强了内胚层发育，而在囊胚晚期进行相同的处理促进了中胚层形成并抑制了内胚层特化。进一步的分析表明，从晚期epiboly到早期的体节发生，pou 2调节额外的发育方面，如大脑区域化，心脏发育和尾巴的形成。我们的研究结果表明，Pou 2功能在脊椎动物发育的多个方面，特别是在中内胚层的中胚层和内胚层的二元决策中，以不同的方式取决于发育阶段。
Zebrafishpou2, encoding the class V POU transcription factor orthologous to mouse Oct‐3/4, has been implicated in different aspects of development, such as dorsoventral patterning, endoderm formation, and brain regionalization, by analyzingpou2mutant embryos. In the present study, we first conducted overexpression ofpou2‐modified genes by mRNA injection, and found thatpou2and its active form (vp‐pou2) augmented mesoderm formation and suppressed endoderm specification, whereas repressivepou2(en‐pou2) affected the formation of the mesoderm and endoderm in a different manner. To avoid complications that might arise from differentpou2functions during the course of development, we used a transgenic line harboring inducibleen‐pou2(HEP), which could function in a dominant‐negative manner. We found that suppressing endogenouspou2byHEPinduction at the mid‐blastula stage enhanced endoderm development at the expense of mesoderm, whereas the same treatment in the late blastulae promoted mesoderm formation and suppressed the endoderm specification. Further analyses usingHEPinduction revealed that, from late epiboly to early somitogenesis,pou2regulated additional developmental aspects, such as brain regionalization, heart development, and tail formation. Our findings suggest that Pou2 functions in multiple aspects of vertebrate development, especially in the binary decision of the mesendoderm to mesoderm and endoderm in different ways depending on the developmental stage.