Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex
Effects of monocular deprivation on the expression pattern of alpha-1 and beta-1 adrenergic receptors in the kitten visual cortex
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DOI：
10.1016/s0168-0102(01)00224-3
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发表时间：
2001-06-01
影响因子：
2.9
通讯作者：
Watanabe, Y
中科院分区：
文献类型：
--
作者：
Nakadate, K;Imamura, K;Watanabe, Y
为了研究肾上腺素能受体是如何通过感觉传出的实验操作来调节的，我们对小猫脑中的α（1）和β（1）肾上腺素能受体进行了免疫组织化学分析。在正常发育中，这些受体在枕叶和额叶皮质的两个半球中表达相似。值得注意的是，单眼剥夺在眼优势可塑性的敏感期显着增加β（1）-肾上腺素能受体的免疫反应性在视觉皮层同侧剥夺的眼睛。单眼剥夺后，在大脑皮质的额叶和顶叶区域以及皮质下结构（包括外侧膝状体核和上级丘）中，β（1）-肾上腺素能受体的免疫反应强度没有增加。此外，这种半球的变化没有发现在α（1）-肾上腺素能受体免疫反应性单眼剥夺。通过微管相关蛋白-2或胶质细胞酸性蛋白双重染色获得的图像的比较表明，增加的免疫反应性位于深层神经元和胶质细胞的顶树突上。这些结果表明，单眼剥夺在眼优势可塑性的敏感期改变β（1）-肾上腺素能受体的免疫反应，包括在胶质细胞。因此，提示胶质细胞中的β（1）肾上腺素能受体在眼优势可塑性的调节中也起重要作用。(C)2001 Elsevier Science爱尔兰有限公司和日本神经科学学会。All rights reserved.
To examine how adrenergic receptors are regulated by experimental manipulation of sensory efferents, we performed immunohistochemical analysis on alpha (1)-, and beta (1)-adrenergic receptors in the brain of kittens. In normal development, these receptors were similarly expressed in both hemispheres of the occipital and frontal cortices. Notably, monocular deprivation during the sensitive period of ocular dominance plasticity significantly increased beta (1)-adrenergic receptor immunoreactivity in the visual cortex ipsilateral to the deprived eye. No increase in the intensity of the immunoreactivity for beta (1)-adrenergic receptors following monocular deprivation was found in the frontal and parietal regions of the cerebral cortex and subcortical structures, including the lateral geniculate nucleus and superior colliculus. Furthermore, such hemispheric change was not found in the alpha (1)-adrenergic receptor immunoreactivity following monocular deprivation. Comparisons of images, obtained by double staining for microtubule-associated protein-2 or glial fibrillary acidic protein, indicated that the increased immunoreactivity was localized on both apical dendrites of deep layer neurons and glial cells. These results indicate that the monocular deprivation during the sensitive period of ocular dominance plasticity modified beta (1)-adrenergic receptor immunoreactivity, including that in glial cells. Therefore, it was suggested that beta (1)-adrenergic receptors in the glial cells also play important roles in the regulation of ocular dominance plasticity. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.