Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells
Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells
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DOI：
10.1021/jf048616l
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发表时间：
2005-03-23
影响因子：
6.1
通讯作者：
Lee, CY
中科院分区：
文献类型：
--
作者：
Heo, HJ;Lee, CY
由于草莓是已知的含有较高浓度的植物化学物质，并具有较高的抗氧化能力之间的共同水果，它们的神经保护活性进行了测试，在体外与过氧化氢处理的PC 12细胞。它们的保护作用和抗氧化能力也与香蕉和橙子进行了比较，这是美国消费量最高的新鲜水果。使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑（MTT）还原测定的细胞活力测试显示草莓酚类物质显著降低氧化应激诱导的神经毒性。由于已知氧化应激也会增加神经元细胞膜的破坏，因此还进行了乳酸脱氢酶（LDH）和台盼蓝排除试验。草莓的细胞保护作用最强。三种水果的相对神经细胞保护活性从高到低依次为草莓>香蕉>橙子。这种保护作用似乎是由于较高的酚类物质含量，包括花青素，草莓中的花青素似乎是主要的贡献者。
Because strawberries are known to contain higher concentrations of phytochemicals and have higher antioxidant capacity among common fruits, their neuroprotective activity was tested in vitro on PC12 cells treated with H2O2. Their protective effect and antioxidant capacity were also compared with those of banana and orange, which are the fresh fruits consumed at highest levels in the United States. The cell viability test using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed that strawberry phenolics significantly reduced oxidative stress-induced neurotoxicity. Because oxidative stress is also known to increase neuronal cell membrane breakdown, lactate dehydrogenase (LDH) and trypan blue exclusion assays were also performed. Strawberry showed the highest cell protective effects among the samples. The overall relative neuronal cell protective activity of three fruits by three tests followed the decreasing order strawberry > banana > orange. The protective effects appeared to be due to the higher phenolic contents including anthocyanins, and anthocyanins in strawberries seemed to be the major contributors.