Bombesin-like immunoreactivity in the lung
Bombesin-like immunoreactivity in the lung
复制标题
肺中的铃蟾肽样免疫反应性
作者：
J. Wharton;Julia M. Polak;S. R. Bloom;M. Ghatei;E. Solcia;Marvin R. Brown;A. Pearse
第一个关于肺含有内分泌细胞的建议是由Feyrter1提出的，当时他提出了弥漫性内分泌或旁分泌系统的概念，该系统由上皮性‘透明细胞’组成。后来证实在人类和其他哺乳动物的肺中存在嗜银细胞2，3并且通过银亲和力、甲醛诱导荧光(FIF)和拥有神经分泌型颗粒证明了其中一些细胞含有胺。它们有不同的名称：Feyrter细胞，Kultschitzky细胞，嗜银荧光颗粒细胞或AFG细胞，肠嗜银细胞7，神经分泌细胞8，肺嗜银细胞9，内分泌或内分泌样细胞10，11，并具有APUD细胞特征10-13。内分泌细胞存在于支气管和细支气管上皮内，既是单个细胞，也是成群的细胞。已证实神经内分泌样细胞组称为神经上皮体4。一些肺内分泌细胞含有5-羟色胺10，14和可能的其他胺，但尚未在它们中发现任何多肽产物。SAID 15提出肺可能产生一种血管活性多肽，随后发现肺产生一种血管紧张素II样多肽16、17和两种血管活性多肽18，尽管这些多肽的细胞来源尚未确定。人胎肺有三种主要的内分泌细胞类型(类型1、类型2和类型3)，这种区别主要基于它们直径110-200 nm的分泌颗粒的形态。肺的2型细胞中的颗粒与肠道和胰腺的P细胞中的颗粒相似。P细胞产物尚未被鉴定，但它可能是蛙皮素样肽20。蛙毒素是从蛙皮中分离出来的一种四肽，对哺乳动物的胃肠道和血管有广泛的作用。肠道的P细胞与肺的某些颗粒细胞之间的相似性表明，人肺中可能存在蛙皮素样多肽，我们在此报告了人胎儿和新生儿肺中蛙皮素样免疫反应的存在。
THE first suggestion that the lung contained endocrine cells was made by Feyrter1 when introducing the concept of a diffuse endocrine or paracrine system, composed of epithelial ‘clear cells’. The presence of argyrophil cells in human and other mammalian lungs was later confirmed2,3 and it was shown that some of these cells contained amines, demonstrated by argent-affinity, formaldehyde-induced fluorescence (FIF), and the possession of neurosecretory type granules. They have variously been termed Feyrter cells4, Kultschitzky cells5, argy-rophil-fluorescent-granulated or AFG cells6, enteroch-romaffin-like cells7, neurosecretory cells8, pulmonary argyrophil cells9, endocrine or endocrine-like cells10,11, and possess APUD cell characteristics10–13. Endocrine cells are present within the bronchial and bronchiolar epithelium both as single elements and as groups of cells. Groups of innervated, endocrine-like cells termed neuroepithelial bodies have been demonstrated4. Some pulmonary endocrine cells contain 5-hydroxytryptamine10,14 and possibly other amines, but no peptide product has yet been identified in them. Said15 has proposed that the lung may produce a vasoactive peptide and it has subsequently been shown that the lung produces an angiotensin II-like peptide16,17 and two vasoactive peptides18, although the cellular origin of these peptides has not been established. Three major types of endocrine cell have been identified in human fetal lung (types 1, 2 and 3), a distinction based largely on the morphology of their secretory granules which are 110–200 nm in diameter12. Granules in the type-2 cell of the lung resemble those in P cells of the gut and pancreas19. The P-cell product has not been identified but it may be a bombesin-like peptide20. Bombesin is a tetra-decapeptide isolated from frog skin21 having a wide range of actions on the mammalian gastrointestinal tract and vasculature22. The similarity between P cells of the gut and certain granulated cells of the lung suggested that a bombesin-like peptide might be present in the human lung, and we report here the presence of bombesin-like immunoreactivity in human fetal and neonatal lung.