The fragilis interferon-inducible gene family of transmembrane proteins is associated with germ cell specification in mice.
The fragilis interferon-inducible gene family of transmembrane proteins is associated with germ cell specification in mice.
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跨膜蛋白的脆弱性干扰素诱导基因家族与小鼠的生殖细胞规范有关。
DOI：
10.1186/1471-213x-3-1
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发表时间：
2003-03-19
影响因子：
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通讯作者：
Surani, M A
中科院分区：
文献类型：
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作者：
Lange, U C;Saitou, M;Western, P S;Barton, S C;Surani, M A
小鼠原始生殖细胞的规范取决于指导性信号事件，其首先作用于赋予上胚层细胞生殖细胞能力，其次，将生殖细胞命运强加于有能力的前体细胞。fragilis是编码跨膜蛋白的干扰素诱导基因，是第一个与生殖细胞能力获得有关的基因。在这里，我们描述了四个额外的fragilis相关的基因，fragilis 2 -5，这是集群在一个68 kb的区域在附近的fragilis基因座的Chr 7。这些基因存在于许多哺乳动物物种中，在人类中也聚集在Chr 11的同线区。在小鼠中，与fragilis接近的fragilis 2和fragilis 3在原始生殖细胞的特化区域中表现出与后者重叠的表达。使用单细胞分析，我们证实，所有这三个脆性相关基因是占主导地位的新生原始生殖细胞，以及在性腺生殖细胞。Fragilis家族干扰素诱导基因与小鼠生殖细胞特化密切相关。此外，它的进化保守性表明它可能在所有哺乳动物中起着关键作用。对这些基因的详细分析也可能阐明干扰素在发育过程中作为信号分子的作用。
Specification of primordial germ cells in mice depends on instructive signalling events, which act first to confer germ cell competence on epiblast cells, and second, to impose a germ cell fate upon competent precursors. fragilis, an interferon-inducible gene coding for a transmembrane protein, is the first gene to be implicated in the acquisition of germ cell competence. Here, we describe four additional fragilis-related genes, fragilis2–5, which are clustered within a 68 kb region in the vicinity of the fragilis locus on Chr 7. These genes exist in a number of mammalian species, which in the human are also clustered on the syntenic region on Chr 11. In the mouse, fragilis2 and fragilis3, which are proximate to fragilis, exhibit expression that overlaps with the latter in the region of specification of primordial germ cells. Using single cell analysis, we confirm that all these three fragilis-related genes are predominant in nascent primordial germ cells, as well as in gonadal germ cells. The Fragilis family of interferon-inducible genes is tightly associated with germ cell specification in mice. Furthermore, its evolutionary conservation suggests that it probably plays a critical role in all mammals. Detailed analysis of these genes may also elucidate the role of interferons as signalling molecules during development.