TRACING OF NEURONAL CONNECTIONS WITH CHOLERA-TOXIN SUBUNIT-B - LIGHT AND ELECTRON-MICROSCOPIC IMMUNOHISTOCHEMISTRY USING MONOCLONAL-ANTIBODIES
TRACING OF NEURONAL CONNECTIONS WITH CHOLERA-TOXIN SUBUNIT-B - LIGHT AND ELECTRON-MICROSCOPIC IMMUNOHISTOCHEMISTRY USING MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0165-0270(88)90167-7
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发表时间：
1988-07-01
影响因子：
3
通讯作者：
BLOMQVIST, A
中科院分区：
文献类型：
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作者：
ERICSON, H;BLOMQVIST, A
霍乱毒素的B亚基被注射到大脑的各个区域，主要是躯体感觉传递结构，作为中枢神经系统的示踪物质。利用小鼠杂交瘤中产生的单抗，用免疫过氧化物酶技术对示踪剂进行定位。这种方法既适用于光学研究，也适用于电子显微镜研究，具有特异性标记和非特异性本底活度之间的高对比度的特点。它提供了树突树的良好逆行标记，因此适合于研究神经元的细胞结构和在超微结构水平上已识别的投射神经元的突触组织。
Subunit B of cholera toxin was used as a tracer substance in the central nervous system after being injected into various brain regions, mainly somatosensory relay structures. The tracer was localized with an immunoperoxidase technique, using monoclonal antibodies raised in mouse hybridomas. This method, which is applicable in both light and electron microscopic studies, is characterized by high contrast between specific labeling and unspecific background activity. It yields excellent retrograde labeling of the dendritic tree and is thus suitable for studying the neuronal cytoarchitecture and, on the ultrastructural level, the synaptic organization of identified projection neurons.