VARIABILITY OF CELL-WALL STRUCTURE AND HYDROCARBON TYPE IN DIFFERENT STRAINS OF BOTRYOCOCCUS-BRAUNII
VARIABILITY OF CELL-WALL STRUCTURE AND HYDROCARBON TYPE IN DIFFERENT STRAINS OF BOTRYOCOCCUS-BRAUNII
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DOI：
10.1111/j.0022-3646.1984.00377.x
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
CHIRAC, C
中科院分区：
文献类型：
--
作者：
BERKALOFF, C;ROUSSEAU, B;CHIRAC, C
对新鲜采集自自然界和实验室培养采集菌株的布氏芽孢杆菌不同样品进行了EM研究，并对其碳氢化合物含量进行了分析。尽管细胞的总体内部结构是相当恒定的，但形成菌落富含碳氢化合物基质的外壁的组织在不同的样本之间有很大的不同。在大多数栽培菌株中，菌落较小，不同的连续外壁仍然明显，所有菌株都含有二烯或三烯烃。相反，大多数采集的样品具有较大的菌落，其基质相当致密，由连续紧密贴合的外壁层的富烃部分形成。这些样品中含有多不饱和烃，即葡萄孢子烯。只有在基质致密的菌株中才能观察到剪除细胞的明确细胞帽。然而，奥斯汀菌株和一些采集的样品是中等的，菌落较小，基质致密，有一定的细胞帽和二烯烃类化合物。因此，碳氢化合物的组成与壁结构的变化没有直接的相关性；然而，尽管在不同的生长阶段进行了分析，但从未在1株菌株中观察到双烯类和类葡萄球状碳氢化合物的出现。因此，存在不同的布氏双胞菌菌株，其中一些合成双烯，另一些合成双烯，似乎极有可能存在。
Different samples of B. braunii Kuetz., freshly collected from nature or laboratory-grown from culture collection strains, were studied by EM and their hydrocarbon content analyzed. Although the general internal structure of the cells was rather constant, the organization of the outer walls forming the hydrocarbon-rich matrix of the colonies differed greatly from one sample to another. In the majority of cultivated strains, the colonies were rather small, the different successive external walls remained distinct and all strains contained dienic or trienic hydrocarbons. In contrast, most of the collected samples possessed large colonies with a rather compact matrix formed by the hydrocarbon-rich part of the successive closely appressed external wall layers. These samples contained polyunsaturated hydrocarbons, i.e., botryococcenes. Well defined cell caps which sheared off the cells were observed only in those strains with a compact matrix. The Austin strain and some collected samples, however, were intermediate with rather small colonies, dense matrix, definite cell caps and dienic hydrocarbons. Thus, the hydrocarbon composition did not correlate directly with the variations in wall structure; however, the occurrence of dienic and botryococcene-like hydrocarbons together in 1 strain was never observed, although analyzed at various stages of growth. Thus, the existence of distinct strains of B. braunii, some synthesizing dienes, others botryococcenes, appears highly probable.