Molecular phylogenetic studies of Brassica, Rorippa, Arabidopsis and allied genera based on the internal transcribed spacer region of 18S-25S rDNA
Molecular phylogenetic studies of Brassica, Rorippa, Arabidopsis and allied genera based on the internal transcribed spacer region of 18S-25S rDNA
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DOI：
10.1006/mpev.1999.0648
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发表时间：
1999-12-01
影响因子：
4.1
通讯作者：
Li, WH
中科院分区：
文献类型：
--
作者：
Yang, YW;Lai, KN;Li, WH
根据18 S-25 S核核糖体DNA内转录间隔区（ITS）序列，分析了菊科4族9属植物的系统发育关系。对7属10种16份材料的ITS区进行了全序列测定。还使用了八个已发表的菊科序列。本研究共包括27个序列，其中4个被发现是假基因。邻接树和简约树都表明这9个属可分为3个类群：（1）拟南芥属、豆蔻属、荠菜属和独行菜属;（2）菜属和碎米荠属;（3）芸苔属、白芥属和萝卜属。在矛盾的建议，豆蔻属和拟南芥被放入一个扩大的部落阿拉伯，我们的数据表明，这两个属是更密切相关的荠属和独行菜族（族Lepidieae）比Rorippa和碎米荠族（族阿拉伯）。此外，我们的数据表明，在族的油菜，萝卜属是更密切相关的B。黑人比B。甘蓝/B.拉帕分支这一结果与几项研究中获得的核数据一致，但与线粒体和叶绿体DNA的RFLP数据相冲突。正如以前的作者所指出的，萝卜可能是B之间的杂交种。nigra和B.甘蓝/B. rapa血统与后者作为母本。(C)北京：科学出版社.
The phylogenetic relationships of nine genera in four tribes of the family Brassicaceae were estimated from the sequences of the internal transcribed spacer region (ITS) of the 18S-25S nuclear ribosomal DNA. The entire ITS region of 16 accessions belonging to 10 species of seven genera was sequenced. Eight published sequences of Brassicaceae were also used. A total of 27 sequences were included in this study; four of them were found to be pseudogenes. Both the neighbor-joining and the parsimony trees suggest that the nine genera can be divided into three groups: (1) Arabidopsis, Cardaminopsis, Capsella, and Lepidium; (2) Rorippa and Cardamine; and (3) Brassica, Sinapis, and Raphanus. In contradiction to the proposal that Cardaminopsis and Arabidopsis be put into an expanded tribe Arabideae, our data show that these two genera are more closely related to Capsella and Lepidium (tribe Lepidieae) than to Rorippa and Cardamine (tribe Arabideae). Further, our data show that within the tribe Brassiceae, Raphanus is more closely related to B. nigra than to the B. oleracea/B. rapa clade. This result is in agreement with the nuclear data obtained in several studies, but is in conflict with the RFLP data of mitochondrial and chloroplast DNA. As pointed out by previous authors, it is possible that Raphanus is a hybrid between the B. nigra and B. oleracea/B. rapa lineages with the latter as the maternal parent. (C) 1999 Academic Press.