Genetic dissection of seed storability using two different populations with a same parent rice cultivar N22.
Genetic dissection of seed storability using two different populations with a same parent rice cultivar N22.
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DOI：
10.1270/jsbbs.65.411
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发表时间：
2015-12
期刊：
影响因子：
2.4
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Lin Q;Wang W;Ren Y;Jiang Y;Sun A;Qian Y;Zhang Y;He N;Hang NT;Liu Z;Li L;Liu L;Jiang L;Wan J
水稻（Oryza sativa L.）的种子耐贮性是一个重要的农艺性状。以 N22（籼稻）为共同亲本的两个分离群体，即。研究了一组来自回交南京35（粳稻）/N22//南京35的122个回交自交系（BIL）和另一个由来自USSR5（粳稻）和N22杂交的189个重组自交系（RIL）组成的群体，以检测控制种子耐贮性的数量性状位点（QTL）。发芽率（GP）用于评价种子在三种不同条件下老化处理后的耐贮性。自然、人工和综合老化治疗。在1、2、5、6和9号染色体上总共鉴定出7个QTL。其中，一个主要QTL qSSn-9在两个群体中很常见。相比之下，在 BIL 中检测到 4 个 QTL（qSSnj-2-1、qSSn-2-2、qSSn-5 和 qSSn-6），在 RIL 中检测到 QTL qSSn-1，这是种子耐贮性的新 QTL。 N22 衍生的等位基因增加了除 qSSnj-2-1 之外的所有位点的种子耐贮性。我们还使用 BIL 中选定的五个具有高耐贮性的品系研究了 QTL 的效果，并用近等基因品系 (NIL) 验证了 qSSn-5。这些结果为金字塔或基于图位的克隆主要 QTL 提供了机会，以提高水稻种子的耐贮性。
Seed storability in rice (Oryza sativa L.) is an important agronomic trait. Two segregating populations with N22 (indica) as a common parent, viz. a set of 122 backcross-inbred lines (BILs) derived from the backcross Nanjing35 (japonica)/N22//Nanjing35 and another population comprising 189 recombinant inbred lines (RILs) from the cross of USSR5 (japonica) and N22, were studied to detect quantitative trait loci (QTL) controlling seed storability. Germination percentage (GP) was used to evaluate seed storability after aging treated under three different conditions, viz. natural, artificial and combined aging treatments. A total of seven QTLs were identified on chromosomes 1, 2, 5, 6 and 9. Among them, a major QTL, qSSn-9, was common in the two populations. In contrast, four QTLs (qSSnj-2-1, qSSn-2-2, qSSn-5 and qSSn-6) were detected in BILs and the QTL qSSn-1 was identified in RILs, which was a new QTL for seed storability. The N22-derived alleles increased the seed storability at all the loci except qSSnj-2-1. We also investigated the effect of QTLs using five selected lines with high storability from BILs and verified qSSn-5 with a near-isogenic line (NIL). These results provide an opportunity for pyramiding or map-based cloning major QTLs for seed storability in rice.