Antibody recognition of Z-DNA.
Antibody recognition of Z-DNA.
复制标题
Z-DNA 的抗体识别。
DOI：
10.1101/sqb.1983.047.01.020
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Stollar,BD
中科院分区：
文献类型：
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作者：
Lafer,EM;Möller,A;Valle,RP;Nordheim,A;Rich,A;Stollar,BD
材料和方法多核苷酸。聚（dG）、聚（dG-dC）、聚（dA-dT）。聚（dA-dT）和聚（dG）。聚-（dC）购自PL Biochemicals，而聚-（A）购自PL Biochemicals。聚（dT）购自Collaborative Research。小牛胸腺DNA从Worthington获得，并进行广泛的氯仿/异戊醇提取。来自幼仓鼠肾细胞的RNA由V. Stollar（Tufts University School of Medicine）提供。Br-聚（dG-dC）。如前所述制备聚（dG-dC）（Lafer等，1981 a）。聚（dG-dmSC）。如Behe和Felsenfeld（1981）所述制备聚（dG-dm-C）。[3H]胸腺嘧啶标记的大肠杆菌DNA由突变株B3制备，如先前Papalian等人所述
MATERIALS AND METHODSPolynucleotides. Poly (dG), poly (dG-dC)'poly (dG-dC), poly (dA-dT). poly (dA-dT), and poly (dG). poly-(dC) were purchased from PL Biochemicals, and poly-(A). poly (dT) was purchased from Collaborative Research. Calf thymus DNA was obtained from Worthington and subjected to extensive chloroform/isoamyl alcohol extraction. RNA from baby hamster kidney cells was provided by V. Stollar (Tufts University School of Medicine). Br-poly (dG-dC). poly (dG-dC) was prepared as described previously (Lafer et al. 1981a). Poly (dG-dmSC). poly (dG-dm~ C) was prepared as described by Behe and Felsenfeld (1981).[3H] Thymidine-labeled Escherichia coli DNA was prepared from a mutant strain B3, as described previously by Papalian et