LEVELS OF NEUTROPHIL ELASTASE AND CATHEPSIN-B ACTIVITIES, AND CYSTATINS IN HUMAN SPUTUM - RELATIONSHIP TO INFLAMMATION
LEVELS OF NEUTROPHIL ELASTASE AND CATHEPSIN-B ACTIVITIES, AND CYSTATINS IN HUMAN SPUTUM - RELATIONSHIP TO INFLAMMATION
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DOI：
10.3109/00365519009089165
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发表时间：
1990-09-01
影响因子：
2.1
通讯作者：
ABRAHAMSON, M
中科院分区：
文献类型：
--
作者：
BUTTLE, DJ;BURNETT, D;ABRAHAMSON, M
对25例支气管扩张症患者的痰液标本进行了髓过氧化物酶、中性粒细胞弹性蛋白酶和组织蛋白酶B的酶法检测，以及半胱氨酸蛋白酶抑制剂A、半胱氨酸蛋白酶抑制剂B、半胱氨酸蛋白酶抑制剂C、半胱氨酸蛋白酶抑制剂S和激肽原的免疫学检测。高髓过氧化物酶和中性粒细胞弹性蛋白酶水平被发现在那些痰样本，目测评估为化脓。还发现这些样本含有高水平的组织蛋白酶B活性和半胱氨酸蛋白酶抑制剂A，但低水平的半胱氨酸蛋白酶抑制剂S和最有效的组织蛋白酶B抑制剂半胱氨酸蛋白酶抑制剂C。相反，髓过氧化物酶和中性粒细胞弹性蛋白酶活性低的痰液样本具有低水平的组织蛋白酶B和半胱氨酸蛋白酶抑制剂A，但具有高水平的半胱氨酸蛋白酶抑制剂C和S。结论：痰液组织蛋白酶B活性与炎症程度及中性粒细胞募集呈正相关。虽然这可能部分是由于组织蛋白酶B抑制剂，特别是胱抑素C的量减少，但理论上的考虑表明，组织蛋白酶B活性的控制中必须涉及抑制剂总水平以外的因素。
Sputum samples from 25 patients with bronchiectasis were assayed enzymatically for myeloperoxidase, neutrophil elastase and cathepsin B, and immunologically for cystatin A, cystatin B, cystatin C, cystatin S and kininogen. High myeloperoxidase and neutrophil elastase levels were found in those sputum samples that were assessed visually to be purulent. These samples were also found to contain high levels of cathepsin B activity and cystatin A, but low levels of cystatin S and of the most effective cathepsin B inhibitor, cystatin C. In contrast, sputum samples that were low in myeloperoxidase and neutrophil elastase activities had low levels of cathepsin B and cystatin A, but high cystatin C and S levels. It is concluded that cathepsin B activity in sputum is positively correlated with the degree of inflammation and neutrophil recruitment. Although this may be due in part to reduced amounts of cathepsin B inhibitors, particularly cystatin C, theoretical considerations suggest that factors other than the gross level of inhibitors must be involved in the control of cathepsin B activity.