CD1d-restricted and TCR-mediated activation of V(alpha)14 NKT cells by glycosylceramides
CD1d-restricted and TCR-mediated activation of V(alpha)14 NKT cells by glycosylceramides
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DOI：
10.1126/science.278.5343.1626
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发表时间：
1997-11-28
期刊：
影响因子：
56.9
通讯作者：
Taniguchi, M
中科院分区：
文献类型：
--
作者：
Kawano, T;Cui, JQ;Taniguchi, M
自然杀伤T（NKT）淋巴细胞表达由V（alpha）14和J（alpha）281基因片段编码的不变T细胞抗原受体（TCR）。具有较长脂肪酰基链（C-26）和鞘氨醇碱基（C-18）的含糖基神经酰胺的α-异头糖被鉴定为该TCR的配体。用氯喹-康卡霉素A或抗CD 1dN（β）8、CD 40/CD 40 L或B7/CTLA-4/CD 28的单克隆抗体处理可消除糖基神经酰胺介导的V（α）14 NKT细胞增殖反应，但不能通过干扰转运蛋白相关蛋白的功能来消除。因此，这种淋巴细胞与T细胞或NK细胞共享不同的识别系统。
Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the V(alpha)14 and J(alpha)281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C-26) and sphingosine base (C-18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of V(alpha)14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1dN(beta)8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.