Quantification of the inhibitory effect of methyl fluoride on methanogenesis in mesophilic anaerobic granular systems
Quantification of the inhibitory effect of methyl fluoride on methanogenesis in mesophilic anaerobic granular systems
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氟化甲烷对中温厌氧颗粒系统产甲烷抑制作用的定量
DOI：
10.1016/j.chemosphere.2011.06.045
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发表时间：
2011-08-01
期刊：
影响因子：
8.8
通讯作者：
Shao, Li-Ming
中科院分区：
文献类型：
--
作者：
Hao, Li-Ping;Lue, Fan;Shao, Li-Ming
在不同浓度下（0- 10%v/v，在气相中），测试了CH 3F对中温厌氧颗粒中甲烷生成的抑制作用，并通过CH 4和CO2的稳定碳同位素特征进行了验证。结果表明，当CH 3F初始浓度达到5%时，抑制效果增强。引起50%代谢抑制的CH 3F浓度为0.32%。当5%CH3F最初加入到顶空时，实现了乙酸裂解产甲烷的完全抑制，总产甲烷活性降低91%。这导致在液体中溶解870 μ M的CH 3 F。这一数值远高于其它天然缺氧非颗粒体系，表明层状颗粒结构影响了抑制效果。氢含量的明显增加表明高浓度的CH_3F（>= 5%）也抑制了氢营养型甲烷生成。随着孵育时间的延长，CH 3F浓度逐渐降低，稳定的抑制作用至少持续6 d。这些结果表明，CH 3F可以用于研究厌氧颗粒系统中的产甲烷过程后，CH 3F的浓度和培养时间的具体抑制乙酸破碎产甲烷仔细确定。在本系统中，CH 3F浓度为5%的建议是最佳的。(C)2011爱思唯尔有限公司版权所有。
The inhibitory effect of CH3F on methanogenesis in mesophilic anaerobic granules was tested at different concentrations (0-10% v/v, in the gas phase) and verified by the stable carbon isotopic signatures of CH4 and CO2. The results showed that the inhibitory effect increased with the initial CH3F concentration up to 5%. The CH3F concentration causing 50% metabolic inhibition was 0.32%. Complete inhibition of acetoclastic methanogenesis with a 91% reduction in total methanogenic activity was achieved when 5% CH3F was initially added to the headspace. which resulted in 870 mu M dissolved CH3F in the liquid. It was much higher than that applied in other natural anoxic non-granular systems, indicating that the layered granular structure influenced the inhibitory effect. The obvious increase in hydrogen content indicated that high concentrations of CH3F (>= 5% )suppressed hydrogenotrophic methanogenesis as well. The stable inhibition lasted for at least 6 d as the CH3F concentration decreased slowly with incubation time. These results suggested that CH3F could be used for investigating methanogenic processes in anaerobic granular systems after the CH3F concentration and incubation time for specific inhibition of acetoclastic methanogenesis were carefully determined. In the present system, CH3F concentration of 5% was suggested to be optimal. (C) 2011 Elsevier Ltd. All rights reserved.