The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.
The effect of histone hyperacetylation on the nuclease sensitivity and the solubility of chromatin.
复制标题
组蛋白超乙酰化对核酸酶敏感性和染色质溶解度的影响。
DOI：
10.1016/s0021-9258(19)69608-0
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
R. Chalkley
中科院分区：
文献类型：
--
作者：
M. Perry;R. Chalkley
我们已经研究了组蛋白超乙酰化后的核酸酶消化的细胞核和随后的分馏的染色体材料在氯化镁的存在下的影响。DNA酶I在离子强度稍高（150-200 mM NaCl）时对超乙酰化核表现出最大敏感性，而微球菌核酸酶在较宽的离子强度范围内对乙酰化核不表现出特异性。在MgCl 2的存在下，用微球菌核酸酶消化的超乙酰化核的分级导致可溶性染色质的量相对于用对照核获得的量大幅增加。这种增加的Mg 2 +-可溶性染色质的产率是由含有极度乙酰化的组蛋白的寡核小体募集到该部分中引起的。这些结果表明，含有高度乙酰化组蛋白的连续核小体可能改变其与自身和与其他核小体相互作用的能力。
We have examined the effects of histone hyperacetylation upon nuclease digestion of nuclei and subsequent fractionation of chromosomal material in the presence of MgCl2. DNase I shows a maximum sensitivity towards hyperacetylated nuclei at somewhat elevated ionic strengths (150-200 mM NaCl), whereas micrococcal nuclease exhibits no specificity for acetylated nuclei over a broad range of ionic strengths. Fractionation in the presence of MgCl2 of hyperacetylated nuclei digested with micrococcal nuclease results in a substantial increase in the amount of soluble chromatin relative to that obtained with control nuclei. This increased yield of Mg2+-soluble chromatin results from the recruitment into this fraction of oligonucleosomes containing extremely hyperacetylated histones. These results suggest that contiguous nucleosomes containing highly acetylated histones may be altered in their ability to interact with themselves and with other nucleosomes.