Isolation and molecular characterization of genotype 1 Japanese encephalitis virus, SX09S-01, from pigs in China
Isolation and molecular characterization of genotype 1 Japanese encephalitis virus, SX09S-01, from pigs in China
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中国猪中基因型 1 日本脑炎病毒 SX09S-01 的分离和分子鉴定
DOI：
10.1186/1743-422x-8-472
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发表时间：
2011-10-14
期刊：
影响因子：
4.8
通讯作者：
Qian, Ping
中科院分区：
文献类型：
--
作者：
Cao, Qi S.;Li, Xiang M.;Qian, Ping
背景猪在日本脑炎病毒（JEV）在蚊子和人类之间传播中发挥着关键作用。 2009年，山西运城某养猪场出现大量仔猪出现病毒性脑炎症状。方法采用BHK-21细胞进行病毒分离。采用免疫组织化学、RT-PCR和间接免疫荧光法对新分离的病毒进行鉴定。对一种分离株（SX09S-01 株）的完整基因组进行了测序和分析。以主要选自中国的24个JEV全长基因组和62个E基因为基础，构建了两棵系统发育树。结果从仔猪中分离到JEV SX09S-01株。序列分析表明，该菌株完整的基因组序列由10965个核苷酸组成，3'非翻译可变区有13个核苷酸缺失。与其他JEV毒株相比，在核苷酸和氨基酸序列水平上的同源性分别为99.1％（XJ69）至74.1％（XZ0934）和99.6％（XJ69）至91.1％（XZ0934）。系统进化树显示SX09S-01株属于基因型I型，与XJ69株亲缘关系最近。结论运城地区乙型脑炎病毒仍存在流行，对猪群中基因型乙脑病毒的主动监测具有重要意义。
BackgroundPigs play a critical role in Japanese encephalitis virus (JEV) transmission between mosquitos and humans. In 2009, lots of piglets developed symptom of viral encephalitis in a pig farm in Yunchen, Shanxi province.MethodsVirus isolation was carried out in BHK-21 cells. Immunohistochemistry, RT-PCR and indirect immunofluorescent assay were used to identify the newly isolated virus. The complete genome of one isolate (SX09S-01 strain) was sequenced and analyzed. Two phylogenetic trees were constructed on the basis of the 24 full-length JEV genomes and 62 E genes mostly selected from China.ResultsJEV SX09S-01 strain was isolated from piglets. Sequence analysis indicates that the completed genome sequences of this strain consists of 10965 nucleotides and there are 13 nucleotides deletion in the 3' nontranslated variable region. Compared with other JEV strains, homology ranges from 99.1% (XJ69) to 74.1% (XZ0934) and 99.6% (XJ69) to 91.1% (XZ0934) on the level of nucleotide and amino acid sequences, respectively. Phylogenetic trees show that SX09S-01 strain belongs to genotype I and it is most closely related to the XJ69 strain.ConclusionsGenotype I of JEV still circulates in Yuncheng and it is thus important for active surveillance on genotype I of JEV from the swine population.