Nicotinic binding sites in rat and mouse brain: comparison of acetylcholine, nicotine, and alpha-bungarotoxin.
Nicotinic binding sites in rat and mouse brain: comparison of acetylcholine, nicotine, and alpha-bungarotoxin.
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发表时间：
1986-11
影响因子：
3.6
通讯作者：
M. Marks;J. Stitzel;E. Romm;J. Wehner;A. C. Collins
中科院分区：
文献类型：
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作者：
M. Marks;J. Stitzel;E. Romm;J. Wehner;A. C. Collins
在从大鼠和小鼠制备的脑组织中比较放射性标记乙酰胆碱（在存在阿托品的情况下测量）、尼古丁和α-银环蛇毒素的结合位点的性质。测试这三个结合位点的以下性质：配体结合的亲和力和密度、竞争性抑制剂的影响、区域分布、用二硫苏糖醇处理的影响和用5，5 '-二硫代双（2-硝基苯甲酸）处理逆转这些影响、热不稳定性、蛋白酶处理的影响和对体内尼古丁长期给药的反应。乙酰胆碱和尼古丁的结合位点的影响相同的所有测量，而α-银环蛇毒素的结合位点的影响的方式不同，从其他两个配体。虽然尼古丁和乙酰胆碱结合的区域分布在大鼠和小鼠大脑之间不同，但这一结合位点的其他特性在两个物种之间非常相似。结果与乙酰胆碱和尼古丁结合到大鼠和小鼠大脑中的相同位点，而α-银环蛇毒素结合到不同位点的提议一致。
The properties of the binding sites for radiolabeled acetylcholine (measured in the presence of atropine), nicotine, and alpha-bungarotoxin were compared in brain tissue prepared from both rat and mouse. These three binding sites were tested for the following properties: affinity and density of ligand binding, effects of competitive inhibitors, regional distribution, effects of treatment with dithiothreitol and the reversal of these effects by treatment with 5,5'-dithiobis(2-nitrobenzoic acid), thermal lability, effects of protease treatment, and response to chronic administration of nicotine in vivo. The binding sites for acetylcholine and nicotine were affected identically for all measurements, whereas the binding site for alpha-bungarotoxin was affected in a manner different from that for the other two ligands. Although the regional distribution of nicotine and acetylcholine binding differed between rat and mouse brain, other properties of this binding site were very similar between the two species. The results are consistent with the proposal that acetylcholine and nicotine bind to the same sites in both rat and mouse brain, whereas alpha-bungarotoxin binds to different sites.