High-performance bioelectrocatalysts created by immobilization of an enzyme into carbon-coated composite membranes with nano-tailored structures
High-performance bioelectrocatalysts created by immobilization of an enzyme into carbon-coated composite membranes with nano-tailored structures
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DOI：
10.1039/c7ta04859a
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发表时间：
2017-10
影响因子：
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通讯作者：
T. Itoh;Yuuta Shibuya;A. Yamaguchi;Yasuto Hoshikawa;O. Tanaike;T. Tsunoda;T. Hanaoka;S. Hamakawa;F. Mizukami;A. Hayashi;T. Kyotani;G. Stucky
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文献类型：
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作者：
T. Itoh;Yuuta Shibuya;A. Yamaguchi;Yasuto Hoshikawa;O. Tanaike;T. Tsunoda;T. Hanaoka;S. Hamakawa;F. Mizukami;A. Hayashi;T. Kyotani;G. Stucky
采用化学气相沉积的方法在大尺寸(40 Mm)的介孔二氧化硅纳米管(F127MST)表面涂覆一层薄层(1-2层石墨烯)，并将制得的F127MST(C/F127MST)直接作为电极。在评价了连续介孔网络的导电性后，将胆红素氧化酶(BOD)负载到C/F127MST的介孔中，形成BOD-C/F127MST。事实上，这种负载过程是有效的，并实现了酶和电极之间的直接电子转移。负载还可以提高BOD的稳定性(可稳定15d)，并可通过改变电势来控制酶反应。因此，我们认为BOD-C/F127MST是一种在各个领域都有应用前景的生物电极。
A large (40 mm ϕ) composite membrane with mesoporous silica nanotubes (F127MST) was coated with a thin carbon layer (1–2 graphene sheets) by carrying out chemical vapor deposition (CVD) using acetylene, and the obtained carbon-coated F127MST (C/F127MST) was used directly as an electrode. After evaluating the electrical conductivity inside the continuous mesopore network, the enzyme bilirubin oxidase (BOD) was loaded into the mesopores of C/F127MST to form BOD–C/F127MST. Indeed, this loading procedure was effective and achieved direct electron transfer between the enzymes and electrodes. The loading also was found to enhance the stability of stored BOD (it was stable for 15 days) and could be used to control the enzymatic reaction by varying the electric potential. We therefore consider BOD–C/F127MST to be a promising candidate as an effective bioelectrode in various fields.