Surface changes in rat spermatozoa during epididymal transit.
Surface changes in rat spermatozoa during epididymal transit.
复制标题
附睾运输过程中大鼠精子的表面变化。
DOI：
10.1095/biolreprod24.2.431
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发表时间：
1981
影响因子：
3.6
通讯作者：
Danzo,BJ
中科院分区：
文献类型：
--
作者：
Olson,GE;Danzo,BJ
用几种不同的方法评价了大鼠附睾头和附睾尾精子质膜的差异。这两个精子群体对凝集素刀豆球蛋白A的凝集力有显着差异，但对小麦胚芽凝集素或蓖麻120的凝集力没有显着差异。刀豆蛋白A受体在头部和尾部精子的表面分布明显不同，头部精子显示ConA结合位点的均匀分布，而尾部精子显示ConA与头部和鞭毛结合的限制性区域。对可用Con A结合部位的直接测量表明，头部的可及部位比尾部精子多15%-25%。头部精子和尾部精子在ConA受体的排列和/或丰度上也不同；主要区别之一是37k的分量大幅增加。通过比较乳酸过氧化物酶催化的碘标记可获得的外向表面蛋白，进一步确定了表面差异；在附睾成熟过程中，特定的高分子量组分的标记减少，而26k和28k的低分子量组分的标记显著增加。讨论了这些变化的可能意义以及产生这些差异的可能机制。
Several different approaches were employed to evaluate differences in the plasma membrane of spermatozoa from the caput and cauda epididymis of the rat. The two sperm populations showed significant differences in agglutinability by the lectin concanavalin A but not by wheat germ agglutinin orRicinus communis120. The surface distribution of concanavalin A receptors between caput and cauda spermatozoa is strikingly different, with caput spermatozoa showing an even distribution of Con A binding sites, whereas cauda sperm showed restricted areas of Con A binding to the head and flagellum. Direct measurement of available Con A binding sites indicated between 15%-25% more accessible sites on caput than on cauda spermatozoa. Caput spermatozoa and cauda spermatozoa also differ in the array and/or abundance of Con A receptors present; one of the major differences is a substantial increase in a component of 37k. Further surface differences were established by comparison of externally oriented surface proteins accessible to lacto-peroxidase-catalyzed iodination; a decreased labeling of specific high molecular weight components and a major increase in labeling of low molecular weight components of 26k and 28k were noted during epididymal maturation. The possible significance of these changes as well as the possible mechanisms responsible for generating these differences are discussed.