INVOLVEMENT OF PROSTAGLANDINS FROM RHEUMATOID SYNOVIUM IN INHIBITION OF ARTICULAR-CARTILAGE METABOLISM
INVOLVEMENT OF PROSTAGLANDINS FROM RHEUMATOID SYNOVIUM IN INHIBITION OF ARTICULAR-CARTILAGE METABOLISM
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DOI：
10.1002/art.1780210807
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发表时间：
1978-01-01
影响因子：
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通讯作者：
MANKIN, HJ
中科院分区：
文献类型：
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作者：
LIPPIELLO, L;YAMAMOTO, K;MANKIN, HJ
兔关节软骨在人类风湿性关节炎滑膜组织培养液中短期孵育，可显著抑制~ 3 H-Gly和Na_（235）SO_4掺入三氯乙酸（TCA）不溶性大分子中，而对预标记软骨基质的降解影响不大。这种抑制作用是时间依赖性的，并且在用吲哚美辛（一种前列腺素合成的特异性抑制剂）培养的类风湿性滑膜培养基中孵育的软骨节段中未观察到。没有吲哚美辛敏感的软骨抑制活性检测培养基中的正常滑膜。大多数抑制活性的醚溶解度和吲哚美辛敏感性表明抑制剂是洋地黄素。
Short term incubations of rabbit articular cartilage in media from cultures of human rheumatoid synovial tissue resulted in marked inhibition of 3H-Gly and Na235SO4 incorporation into trichloroacetic acid (TCA) insoluble macromolecules by the cartilage segments with little effect on degradation of prelabeled cartilage matrix. This inhibition was time-dependent and not observed in cartilage segments incubated in media derived from rheumatoid synovia cultured with indomethacin (a specific inhibitor of prostaglandin synthesis). No indomethacin-sensitive cartilage inhibitory activity was detectable in media from cultures of normal synovia. The ether solubility of the majority of the inhibitory activity and the indomethacin sensitivity suggest that the inhibitor(s) are prostaglandins.