New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.
New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.
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DOI：
10.1039/c5np00085h
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发表时间：
2016-02
影响因子：
11.9
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Luo Y;Enghiad B;Zhao H
天然产物支架仍然是人类治疗的主要来源和灵感。然而，在后基因组时代产生天然产物通常需要在异源宿主中重建相应的生物合成基因簇。在新兴的合成生物学和代谢工程领域，大量的努力致力于开发具有更高效率、保真度和模块化的DNA组装技术，以及具有更高生产力和产量的异源表达系统。在这里，我们描述了DNA组装和宿主工程的最新进展，并强调其在天然产物发现和工程中的应用。
Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.