Streptomyces associated with a marine sponge Haliclona sp.; biosynthetic genes for secondary metabolites and products
Streptomyces associated with a marine sponge Haliclona sp.; biosynthetic genes for secondary metabolites and products
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DOI：
10.1111/j.1462-2920.2010.02337.x
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发表时间：
2011-02-01
影响因子：
5.1
通讯作者：
Shin-ya, Kazuo
中科院分区：
文献类型：
--
作者：
Khan, Shams Tabrez;Komaki, Hisayuki;Shin-ya, Kazuo
陆生放线菌是生物活性化合物的主要来源；然而，新化合物发现的迅速减少强烈需要新的研究方法。一种方法是从海洋生境中筛选放线菌，特别是链霉菌属的成员。本属在海绵体Haliclona sp.中的存在用培养依赖和不依赖技术进行了研究。16S rRNA基因克隆文库分析显示，海绵样品中存在多种链霉菌。除了优势属链霉菌外，还利用4种不同的培养基分离到了6个不同属的成员。还分离出5个系统发育上的新菌株，每个菌株代表链霉菌属的一个新种。多相研究表明，这两个菌株的分类作为新种提出。在菌株中寻找新化合物的产生和次生代谢产物的生物合成基因，发现了7个具有独特序列的新化合物和生物合成基因。在这些化合物中，JBIR-43对癌细胞表现出细胞毒活性。JBIR-34和-35由于其独特的化学结构而特别有趣。据我们所知，这是第一次详细介绍从海绵中分离放线菌和从这些菌株中分离出新的次级代谢物的综合研究。
P>Terrestrial actinobacteria have served as a primary source of bioactive compounds; however, a rapid decrease in the discovery of new compounds strongly necessitates new investigational approaches. One approach is the screening of actinobacteria from marine habitats, especially the members of the genus Streptomyces. Presence of this genus in a marine sponge, Haliclona sp., was investigated using culture-dependent and -independent techniques. 16S rRNA gene clone library analysis showed the presence of diverse Streptomyces in the sponge sample. In addition to the dominant genus Streptomyces, members of six different genera were isolated using four different media. Five phylogenetically new strains, each representing a novel species in the genus Streptomyces were also isolated. Polyphasic study suggesting the classification of two of these strains as novel species is presented. Searching the strains for the production of novel compounds and the presence of biosynthetic genes for secondary metabolites revealed seven novel compounds and biosynthetic genes with unique sequences. In these compounds, JBIR-43 exhibited cytotoxic activity against cancer cell lines. JBIR-34 and -35 were particularly interesting because of their unique chemical skeleton. To our knowledge, this is the first comprehensive study detailing the isolation of actinobacteria from a marine sponge and novel secondary metabolites from these strains.