Persistence of antibodies to West Nile virus nonstructural protein 5
Persistence of antibodies to West Nile virus nonstructural protein 5
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DOI：
10.1016/j.jcv.2008.03.025
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发表时间：
2008-09-01
影响因子：
8.8
通讯作者：
Busch, Michael P.
中科院分区：
文献类型：
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作者：
Prince, Harry E.;Lape-Nixon, Mary;Busch, Michael P.
背景：由于抗西尼罗病毒（WNV）膜前/包膜（preM/E）重组蛋白的IgM抗体可能持续1年以上，因此区分最近和过去的西尼罗病毒感染的检测将是有用的。对单个患者发表的研究结果表明，存在针对西尼罗河病毒非结构蛋白5 （NS5）的抗体表明最近感染。目的：比较在献血时病毒血症的献血者血浆样本中WNV NS5抗体和preM/E IgM的持久性。研究设计：使用微球免疫分析法对来自35个病毒血症供者的随访血浆样本进行了西尼罗河病毒NS5抗体检测，并与使用酶联免疫吸附法（ELISA）在相同样本中检测的西尼罗河病毒preM/E IgM抗体进行了比较。结果：随访90 +/- 14天，20/26（77%）供者NS5抗体阳性；6/25（24%）在180±27天呈阳性。3/23（13%）在365 +/- 55天呈阳性。preM/E I-M抗体的可比值分别为77%、32%和17%。结论：WNV NS5抗体在血浆中的持久性与preM/E IgM抗体相似。西尼罗河病毒NS5抗体不能用于区分最近和过去的西尼罗河病毒感染。(C) 2008 Elsevier B.V.版权所有
Background: Because IgM antibody against West Nile virus (WNV) pre-membrane/envelope (preM/E) recombinant protein may persist for more than 1 year, an assay distinguishing recent from past WNV infection would be useful. Published findings for a single patient suggest that the presence of antibody against WNV nonstructural protein 5 (NS5) indicates recent infection.Objectives: To compare the persistence of WNV NS5 antibodies and preM/E IgM using plasma samples from blood donors who were viremic at the time of donation.Study design: Follow-up plasma samples from 35 viremic donors were tested for WNV NS5 antibodies using a microsphere immunoassay, and compared to WNV preM/E IgM antibodies determined on the same samples using an enzyme-linked immunosorbent assay (ELISA).Results: At 90 +/- 14 days of follow-up, 20/26 donors (77%) were positive for NS5 antibodies; 6/25 (24%) were positive at 180 +/- 27 days. and 3/23 (13%) were positive at 365 +/- 55 days. The comparable values for preM/E I-M antibodies were 77%, 32% and 17%, respectively.Conclusion: Persistence of WNV NS5 antibody in plasma is similar to that of preM/E IgM antibody. WNV NS5 antibody cannot be used to distinguish recent from past WNV infection. (C) 2008 Elsevier B.V. All rights reserved.