Simultaneous Administration of Fluoride and Selenite Regulates Proliferation and Apoptosis in Murine Osteoblast-like MC3T3-E1 Cells by Altering Osteoprotegerin
Simultaneous Administration of Fluoride and Selenite Regulates Proliferation and Apoptosis in Murine Osteoblast-like MC3T3-E1 Cells by Altering Osteoprotegerin
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DOI：
10.1007/s12011-011-9130-x
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发表时间：
2011-07
影响因子：
3.9
通讯作者：
G. Ren;Kai Wang;Ruilong Chang;Yong Su;Jianfang Wang;Jingliang Su;B. Han
中科院分区：
文献类型：
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作者：
G. Ren;Kai Wang;Ruilong Chang;Yong Su;Jianfang Wang;Jingliang Su;B. Han
受体活化剂核因子κ B配体（RANKL）及其诱饵受体骨保护素（OPG）对于维持骨形成和骨吸收之间的平衡非常重要。然而，微量元素对这些因子的调节作用尚不清楚。在这项研究中，我们使用小鼠成骨细胞样MC 3 T3-E1细胞来检查氟化钠（NaF）和/或亚硒酸钠（Na 2SeO 3）对OPG/RANKL系统的影响。用OPG或RANKL siRNA处理MC 3 T3-E1细胞（或不处理），随后分为对照组和5个实验组，将其暴露于不同浓度的NaF和/或Na 2SeO 3，随后在24 h进行分析。特别是，我们检查了细胞活力，OPG和RANKL mRNA和蛋白质表达，caspase-3活性，以及各种细胞组的细胞周期。总之，我们的研究结果表明，氟化钠和/或亚硒酸钠的管理影响OPG在成骨样MC 3 T3-E1细胞的表达，从而有助于由OPG诱导的增殖和凋亡。
The receptor activator nuclear factor kappa-B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important for maintaining the balance between bone formation and resorption. However, the regulation of microelements on these factors remains unclear. In this study, we used murine osteoblast-like MC3T3-E1 cells to examine the impact of sodium fluoride (NaF) and/or sodium selenite (Na2SeO3) on the OPG/RANKL system. MC3T3-E1 cells were treated with OPG or RANKL siRNA (or left untreated), and subsequently divided into a control group and five experimental groups, which were exposed to different concentrations of NaF and/or Na2SeO3, and subsequently analysed at 24 h. In particular, we examined cell viability, OPG and RANKL mRNA and protein expression, caspase-3 activity, and the cell cycle of the various cell groups. In summary, our findings suggest that the administration of NaF and/or Na2SeO3 affects the expression of OPG in osteoblast-like MC3T3-E1 cells, thereby contributing to the proliferation and apoptosis induced by the OPG.