Abnormal migration phenotype of mitogen-activated protein kinase-activated protein kinase 2-/- neutrophils in Zigmond chambers containing formyl-methionyl-leucyl-phenylalanine gradients
Abnormal migration phenotype of mitogen-activated protein kinase-activated protein kinase 2-/- neutrophils in Zigmond chambers containing formyl-methionyl-leucyl-phenylalanine gradients
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DOI：
10.4049/jimmunol.167.7.3953
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发表时间：
2001-10-01
影响因子：
4.4
通讯作者：
Huang, CK
中科院分区：
文献类型：
--
作者：
Hannigan, MO;Zhan, LJ;Huang, CK
使用延时视频显微镜和共聚焦成像研究野生型（WT）和促分裂原活化蛋白激酶活化蛋白激酶2（MK2）（-/-）小鼠中性粒细胞在含有fMLP梯度的Zigmond室中的迁移。极化WT中性粒细胞的共聚焦图像显示从中性粒细胞的前部到后部区域的磷酸-MK2的细胞内梯度。与WT中性粒细胞相比，MK2（-/-）中性粒细胞表现出部分方向性丧失，但迁移速度更快。免疫印迹实验表明，较低的蛋白质水平的p38丝裂原活化蛋白激酶和fMLP诱导的细胞外信号相关激酶磷酸化的MK2（-/-）中性粒细胞的损失。这些结果表明，MK2在调节中性粒细胞迁移中起重要作用，并可能影响其他信号分子。
Time-lapsed video microscopy and confocal imaging were used to study the migration of wild-type (WT) and mitogen-activated protein kinase-activated protein kinase 2 (MK2)(-/-) mouse neutrophils in Zigmond chambers containing fMLP gradients. Confocal images of polarized WT neutrophils showed an intracellular gradient of phospho-MK2 from the anterior to the posterior region of the neutrophils. Compared with WT neutrophils, MK2(-/-) neutrophils showed a partial loss of directionality but higher migration speed. Immunoblotting experiments showed a lower protein level of p38 mitogen-activated protein kinase and a loss of fMLP-induced extracellular signal-related kinase phosphorylation in MK2(-/-) neutrophils. These results suggest that MK2 plays an important role in the regulation of neutrophil migration and may also affect other signaling molecules.