Measurement of bisphenol A concentrations in human colostrum
Measurement of bisphenol A concentrations in human colostrum
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DOI：
10.1016/j.chemosphere.2006.06.073
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Nozawa, Ryushi
中科院分区：
文献类型：
--
作者：
Kuruto-Niwa, Ryoko;Tateoka, Yumiko;Nozawa, Ryushi
双酚A （BPA）是一种雌激素内分泌干扰化学物质，据报道会影响胚胎并改变其产后发育。在本研究中，我们通过竞争性酶联免疫吸附试验（ELISA）测量了人初乳中BPA的浓度，目的是了解日本静冈市人母乳中BPA负荷的现状。在分娩后三天内，从101名健康母亲身上采集了人类初乳样本。经乙腈萃取和固相萃取柱纯化后，采用ELISA法测定初乳样品的BPA浓度。101份样品中检测到的BPA浓度范围为1 ~ 7 ng ml（-1）。BPA的平均浓度为3.41 +/- 0.13（平均值+/- SD） ng ml（-1）。这是第一次证明BPA在人类初乳中的浓度。在之前的一项研究中，初乳中的BPA浓度高于从健康女性身上获得的血清样本。在我们的研究中，BPA的浓度之间没有显著的相关性。在初乳和母亲的年龄和胎次。(c) 2006 Elsevier Ltd.版权所有。
Bisphenol A (BPA), an estrogenic endocrine disrupting chemical, has been reported to affect embryos and alter their postnatal development. In the present study, we measured the concentrations of BPA in human colostrum by a competitive enzyme-linked immunosorbent assay (ELISA) with the aim of understanding the present status of BPA burden in human breast milk in Shizuoka, Japan. Human colostral samples were collected from 101 healthy mothers within three days after delivery. The BPA concentrations of colostral samples were estimated by ELISA after the acetonitrile extraction and solid phase extraction column purification. BPA in 101 samples was detected in the concentration range of 1-7 ng ml(-1). The mean concentration of BPA was 3.41 +/- 0.13 (mean +/- SD) ng ml(-1). This is the first demonstration as to what BPA concentrations are in human colostrum. The BPA concentrations in colostrum were higher than those in blood sera samples obtained from healthy women in a previous study. In our study, there was no significant correlation between the concentrations of BPA. in colostrum and the age and parity of mothers. (c) 2006 Elsevier Ltd. All rights reserved.