Image-Derived Input Function from the Vena Cava for 18F-FDG PET Studies in Rats and Mice
Image-Derived Input Function from the Vena Cava for 18F-FDG PET Studies in Rats and Mice
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DOI：
10.2967/jnumed.113.127381
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发表时间：
2014-08-01
影响因子：
9.3
通讯作者：
Gruetter, Rolf
中科院分区：
文献类型：
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作者：
Lanz, Bernard;Poitry-Yamate, Carole;Gruetter, Rolf
由于啮齿类动物的总血容量小且相关的取血困难，动脉输入功能的测量是定量F-18-FDG PET研究的一个限制性方面。方法：在本研究中，我们利用最新的专用小动物扫描仪的高空间分辨率，通过腔静脉从Sprague-Dawley大鼠（n = 4）和C57BL/6小鼠（n = 5）的F-18-FDG PET图像中提取输入函数。在大鼠实验中，采用外置微容量血液计数器作为测定动脉输入功能的参考，对图像衍生输入功能（IDIF）方法进行验证，并通过额外的人工采血来确认其测量结果。对示踪剂在静脉腔和动脉树之间的血液分散进行校正。此外，我们还进行了模拟研究，以探讨不同的IDIF提取方法对测定的脑葡萄糖代谢率（CMRGlc）的影响。在小鼠测量中，使用IDIF计算CMRGlc，并使用Patlak方法将其与先前报道的值进行比较。结果：使用室室建模或Patlak方法，从静脉腔中提取的IDIF显示出可靠的CMRGlc测定，即使没有进行大规模弥散校正或采血，与参考数据相比，CMRGlc的低估为7% +/- 16%。使用这种方法在小鼠实验中，我们测量到大脑皮层的脑代谢率为0.22 +/- 0.10 μ mol/g/min（平均SD），与先前在小鼠大脑中的F-18-FDG研究结果很好地一致。在大鼠实验中，使用单个手工血液样本对IDIF进行弥散校正和对IDIF进行额外缩放，从而优化了CMRGlc的测定，低估了6% - 7%。结论：因此，静脉洞穴时间活动曲线是测量大鼠和小鼠F-18-FDG输入功能的一种微创替代方法，没有重复采血相关的并发症。
Measurement of arterial input function is a restrictive aspect for quantitative F-18-FDG PET studies in rodents because of their small total blood volume and the related difficulties in withdrawing blood. Methods: In the present study, we took advantage of the high spatial resolution of a recent dedicated small-animal scanner to extract the input function from the F-18-FDG PET images in Sprague-Dawley rats (n = 4) and C57BL/6 mice (n = 5), using the vena cava. In the rat experiments, the validation of the image-derived input function (IDIF) method was made using an external microvolumetric blood counter as reference for the determination of the arterial input function, the measurement of which was confirmed by additional manually obtained blood samples. Correction for tracer bolus dispersion in blood between the vena cave and the arterial tree was applied. In addition, simulation studies were undertaken to probe the impact of the different IDIF extraction approaches on the determined cerebral metabolic rate of glucose (CMRGlc). In the mice measurements, the IDIF was used to compute the CMRGlc, which was compared with previously reported values, using the Patlak approach. Results: The presented IDIF from the vena cave showed a robust determination of CMRGlc using either the compartmental modeling or the Patlak approach, even without bolus dispersion correction or blood sampling, with an underestimation of CMRGlc of 7% +/- 16% as compared with the reference data. Using this approach in the mice experiments, we measured a cerebral metabolic rate in the cortex of 0.22 +/- 0.10 mu mol/g/min (mean SD), in good agreement with previous F-18-FDG studies in the mouse brain. In the rat experiments, dispersion correction of the IDIF and additional scaling of the IDIF using a single manual blood sample enabled an optimized determination of CMRGlc, with an underestimation of 6% 7%. Conclusion: The vena cave time activity curve is therefore a minimally invasive alternative for the measurement of the F-18-FDG input function in rats and mice, without the complications associated with repetitive blood sampling.