Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure
Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure
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DOI：
10.1039/d1cc00876e
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发表时间：
2021-05-07
影响因子：
4.9
通讯作者：
Endo, Masayuki
中科院分区：
文献类型：
--
作者：
Abe, Katsuhiko;Sugiyama, Hiroshi;Endo, Masayuki
我们证明了使用具有Cas9核酸酶的DNA折纸结构的光控序列选择性dsDNA切割。掺入环形DNA折纸内的Cas9的活性被完全抑制。在光照射后，Cas9被释放，并且其选择性dsDNA切割的活性被恢复。
We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.