Chlorhexidine release and antibacterial properties of chlorhexidine-incorporated polymethyl methacrylate-based resin cement
Chlorhexidine release and antibacterial properties of chlorhexidine-incorporated polymethyl methacrylate-based resin cement
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DOI：
10.1002/jbm.b.31633
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发表时间：
2010-07-01
影响因子：
3.4
通讯作者：
Tay, F. R.
中科院分区：
文献类型：
--
作者：
Hiraishi, N.;Yiu, C. K. Y.;Tay, F. R.
本研究评估了从由Super - Bond C&B（太阳医疗公司）制备的含氯己定的实验性聚甲基丙烯酸甲酯（PMMA）基树脂水门汀中释放氯己定的情况，并检测了其对变形链球菌和粪肠球菌的抗菌活性。将二醋酸氯己定添加到PMMA聚合物中，以使氯己定浓度分别达到0.0、1.0、2.0、3.0和4.0 wt%。将含氯己定的固化树脂圆盘在37℃的蒸馏水中浸泡5周，通过高效液相色谱法分析氯己定的释放情况。使用琼脂扩散试验检测新混合的树脂水门汀的抗菌效果。对于直接接触试验，在微量滴定板的孔（n = 6）中涂上水门汀。在将细菌悬液直接置于固化的水门汀上之前，将涂有水门汀的孔老化长达3周。含3.0%和4.0%氯己定的水门汀在5周内都有氯己定释放；然而，超过98%的氯己定保留在树脂基质中。含1.0%和2.0%氯己定的水门汀分别在1周和2周时未检测到释放。琼脂扩散试验未能检测到对粪肠球菌的抗菌作用，而直接接触试验显示含3.0%和4.0%氯己定的水门汀在2周内对每种微生物都有抗菌作用。含3.0%和4.0%氯己定的树脂水门汀具有长达2周的氯己定持续释放和抗菌特性。（C）2010威利期刊公司。《生物医学材料研究B部分：应用生物材料》94B：134 - 140，2010年。
This study evaluated chlorhexidine release from experimental, chlorhexidine-incorporated polymethyl methacrylate (PMMA)-based resin cements prepared from Super-Bond C&B (Sun Medical) and examined the antimicrobial activity against Streptococcus mutans and Enterococcus faecalis. Chlorhexidine diacetate was added into PMMA polymer to obtain chlorhexidine concentration of 0.0, 1.0, 2.0, 3.0, and 4.0 wt %. Chlorhexidine-incorporated, cured resin disks were immersed in distilled water at 37 C for 5 weeks, and the chlorhexidine release was analyzed by high-performance liquid chromatography. The antibacterial effect of freshly mixed resin cements was examined using the agar diffusion test. For the direct contact test, the wells In = 6) of microtiter plates were coated with cements. The coated wells were aged up to 3 weeks prior to the placement of bacterial suspensions directly on cured cements. The 3.0 and 4.0% chlorhexidine-incorporated cement exhibited chlorhexidine release for 5 weeks; however, more than 98% of chlorhexidine was retained in resin matrix. No release was detected from the 1.0 and 2.0% incorporated cement at 1 week and 2 weeks, respectively. The agar diffusion test failed to detect antibacterial effects against Enterococcus faecalis, whereas the direct contact test revealed the antibacterial effect of 3.0 and 4.0% incorporated cements against each microbe for 2 weeks. The 3.0 and 4.0% chlorhexidine-incorporated resin cement possessed prolonged chlorhexidine release and antibacterial properties for 2 weeks. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 94B: 134-140, 2010.