Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi
Transcriptional and metabolic profiling of grape (Vitis vinifera L.) leaves unravel possible innate resistance against pathogenic fungi
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DOI：
10.1093/jxb/ern187
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发表时间：
2008-09-01
影响因子：
6.9
通讯作者：
Pais, Maria Salome
中科院分区：
文献类型：
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作者：
Figueiredo, Andreia;Fortes, Ana Margarida;Pais, Maria Salome
葡萄藤属（葡萄属sp.）在世界范围内，真菌是影响其栽培和经济效益的主要病原体。知识的复杂性机制负责抗真菌感染的品种，如丽晶，是必要的战略来定义，这将提高高敏感作物品种的抗性。通过cDNA微阵列、实时定量PCR和核磁共振波谱分析了葡萄属葡萄品种Regent和Trincadeira（分别对真菌具有抗性和敏感性）的转录和代谢谱。通过转录组和代谢组学分析获得的数据集的整合揭示了两个品种之间的转录物和代谢物的差异。这些差异可能与瑞金特对霉菌的先天抗性有关。与胁迫和防御相关的几种转录物，即枯草杆菌蛋白酶样蛋白酶、苯丙氨酸解氨酶、S-腺苷甲硫氨酸合酶、WD-重复蛋白样和J2 P，在Regent中上调，表明该品种具有内在的抗性能力。代谢谱显示，肌醇和咖啡酸等化合物的积累，已知这些化合物可赋予对真菌的抗性。转录本和代谢产物检测的差异进行了讨论，在植物防御病原体攻击的代谢途径和它们可能的作用，以及它们的潜在利益，以区分抗性和敏感葡萄藤品种。
Grapevine species (Vitis sp.) are prone to several diseases, fungi being the major pathogens compromising its cultivation and economic profit around the world. Knowledge of the complexity of mechanisms responsible for resistance to fungus infection of cultivars, such as Regent, is necessary for strategies to be defined which will improve resistance in highly susceptible crop species. Transcript and metabolic profiles of the Vitis vinifera cultivars Regent and Trincadeira (resistant and susceptible to fungi, respectively) were analysed by cDNA microarray, quantitative real-time PCR, and nuclear magnetic resonance spectroscopy. The integration of datasets obtained through transcriptome and metabolome analysis revealed differences in transcripts and metabolites between both cultivars. These differences are probably associated with the innate resistance of Regent towards the mildews. Several transcripts related to stress and defence, namely a subtilisin-like protease, phenylalanine ammonia lyase, S-adenosylmethionine synthase, WD-repeat protein like, and J2P, were up-regulated in Regent suggesting an intrinsic resistance capability of this cultivar. A metabolic profile revealed an accumulation of compounds such as inositol and caffeic acid, which are known to confer resistance to fungi. The differences in transcripts and metabolites detected are discussed in terms of the metabolic pathways and their possible role in plant defence against pathogen attack, as well as their potential interest to discriminate among resistant and susceptible grapevine cultivars.