Characterization of the major parathyroid hormone target cell in the endosteal metaphysis of rat long bones
Characterization of the major parathyroid hormone target cell in the endosteal metaphysis of rat long bones
复制标题
大鼠长骨骨内干骺端主要甲状旁腺激素靶细胞的表征
DOI：
10.1002/jbmr.5650051008
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发表时间：
1990
影响因子：
6.2
通讯作者：
D. Goltzman
中科院分区：
文献类型：
--
作者：
Marie F. Rouleau;Jane Mitchell;D. Goltzman
最近的研究表明，在大鼠长骨的骨内膜干骺端，甲状旁腺激素（PTH）在体内的竞争性结合主要发生在骨小梁间组织的一个不同于分化成骨细胞的细胞上。在本报告中，我们进一步表征了这种细胞，称为甲状旁腺激素靶（PT）细胞，通过光镜和电子显微镜，使用放射自显影和组织化学技术。这些研究表明，PT细胞是一种单核细胞，其大细胞体有时位于分化成骨细胞簇之间，也位于骨小梁组织的其他区域。它的长细胞质突起从骨基质穿过骨小梁间区向血管结构延伸，与内皮的各种细胞交错。起源于高尔基体系统的独特管状结构，通常与长线粒体和糖原颗粒有关，贯穿于PT细胞的细胞质过程。基于其结合[3H]胸腺嘧啶的能力，PT细胞似乎分裂得相当缓慢。偶尔发现的具有PT细胞和分化成骨细胞超微结构特征的杂交细胞，以及碱性磷酸酶活性的组织化学证据表明，PT细胞属于成骨细胞谱系。因此，这些研究从形态学上定义了甲状旁腺瘤的主要骨靶细胞，尽管属于成骨细胞谱系，但不是分化的成骨细胞，并提供了体内证据，证明“成骨细胞表型”的特征并不局限于单一的骨细胞类型。
The majority of in vivo competitive binding of parathyroid hormone (PTH) in the endosteal metaphysis of rat long bones was recently shown to be localized in the intertrabecular tissue to a cell that is distinct from a differentiated osteoblast. In the present report we have further characterized this cell, termed a parathyroid hormone target (PT) cell, by light and electron microscopy using radioautography and histochemical techniques. These studies demonstrate that the PT cell is a mononuclear cell with a large cell body located at times between clusters of differentiated osteoblasts, as well as in other regions of the intertrabecular tissue. Its long cytoplasmic processes extend from the bone matrix through the intertrabecular region toward vascular structures, interdigitating with various cells of the endosteum. A distinctive tubular structure originating in the Golgi system and often associated with long mitochondria and glycogen particles extends throughout the cytoplasmic processes of the PT cell. Based on its capacity to incorporate [3H]thymidine, the PT cell appears to divide rather slowly. The identification of occasional hybrid cells with ultrastructural features of both the PT cell and the differentiated osteoblast and the presence of histochemical evidence for alkaline phosphatase activity suggest that the PT cell is of the osteoblast lineage. These studies therefore morphologically define a major osseous target cell for PTH that, although of the osteoblast lineage, is not a differentiated osteoblast and provide in vivo evidence that characteristics of the “osteoblast phenotype” are not restricted to a sole osseous cell type.